| 牛皮蝇Hypodermin B 基因的克隆及原核表达载体构建 |
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| 引用本文： | 唐立刚,杨东英,殷宏,罗建勋,欧阳五庆. 牛皮蝇Hypodermin B 基因的克隆及原核表达载体构建[J]. 中国牛业科学, 2004, 30(2): 4-6 |
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| 作者姓名： | 唐立刚 杨东英 殷宏 罗建勋 欧阳五庆 |
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| 作者单位： | 西北农林科技大学动物科技学院,陕西杨凌,712100;中国农业科学院兰州兽医研究所,甘肃兰州,730046;中国农业科学院兰州兽医研究所,甘肃兰州,730046;西北农林科技大学动物科技学院,陕西杨凌,712100 |
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| 基金项目： | 国家863:项目编号为:2001-AA249081 |
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| 摘 要： | 参照牛皮蝇Hypodermin B(HB)基因的核苷酸序列，设计一对引物，以牛皮蝇总RNA为模板利用RT—PCR扩增牛皮蝇HB基因，并对扩增基因进行克隆测序。序列分析表明，克隆的牛皮蝇HA基因与GenBank中已经登录的核苷酸和氨基酸序列的同源性分别为97．2％和99．3％。同时，将该基因与原核表达载体．PGEX-4T-1连接，构建并获得阳性重组表达载体。
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| 关 键 词： | 牛皮蝇 Hypodermin B基因 克隆 重组表达载体 |
| 文章编号： | 1001-9111(2004)02-0004-03 |
| 修稿时间： | 2003-12-15 |
Cloning and Prokaryotic Expression Vector Construction of Hypodermin B.Gene of H.Bovis |
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| TANG Li-gang{,},YANG Dong-ying{,},YIN Hong,LUO Jian-xun,OUYANG-Wuqing. Cloning and Prokaryotic Expression Vector Construction of Hypodermin B.Gene of H.Bovis[J]. China Cattle Science, 2004, 30(2): 4-6 |
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| Authors: | TANG Li-gang{ } YANG Dong-ying{ } YIN Hong LUO Jian-xun OUYANG-Wuqing |
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| Affiliation: | TANG Li-gang~{1,2},YANG Dong-ying~{1,2},YIN Hong~2,LUO Jian-xun~2,OUYANG-Wuqing~1 |
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| Abstract: | Hypodermin B. gene of H. Bovis was firstly amplified by RT-PCR with the primers designed on the nucleotide sequence of Hypodermin B. gene and the total RNA of H. Bovis, then the Hypodermin B. gene was cloned and sequenced. Results showed that the homologies of the Cloned Hypodermin B. gene nucleotide and amino acid sequences and the relative sequences in GenBank were 97.2 percent and 99.3 percent, respectively. The positive recombined expression vector of Hypodermin B. gene was finally constructed with the prokaryotic expression vector of PGEX-4T-1 and identified. |
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| Keywords: | H. Bovis Hypodermin B gene Cloning Recombined expression vector |
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