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| 新疆多浪羊白细胞介素8 cDNA的克隆测序及其变异SNP的分析 | |
| 引用本文： | 杨威华,左文斌,潘辉,贺艳艳,刘书东,徐丽君,王娟娟,李莲瑞.新疆多浪羊白细胞介素8 cDNA的克隆测序及其变异SNP的分析[J].塔里木农垦大学学报,2011,23(1):1-8. |
| 作者姓名： | 杨威华 左文斌 潘辉 贺艳艳 刘书东 徐丽君 王娟娟 李莲瑞 |
| 作者单位： | 1. 塔里木大学动物科学学院,新疆,阿拉尔,843300 2. 塔里木大学生命科学学院,新疆,阿拉尔,843300 3. 新疆生产建设兵团塔里木畜牧科技重点实验室,新疆,阿拉尔,843300 |
| 摘 要： | 采集多浪羊的血液,分离白细胞,提取总RNA,用白细胞介素8（IL-8）的引物进行两步法的RT-PCR扩增、克隆与酶切鉴定,测序后进行序列比对,与NM_001009401.1同源性高达99%,初步判定为多浪羊的IL-8编码全长cDNA。通过序列比对,发现多浪羊IL-8的基因起始密码子上游第七个碱基发生突变,在NM_001009401.1中是T,而在测序的多浪羊的IL-8中是C。对该测序序列进行数据库搜索和序列比对,发现该碱基变异很独特,并对该变异可能对mRNA蛋白翻译的影响进行了初步的分析。 |
| 关 键 词： | 多浪羊 白细胞介素8 SNP 序列分析 mRNA二级结构 |
Cloning and Sequencing of the Interleukin 8 of Xinjiang Duolang-sheep and Analysis of its Mutant Nucleotide |
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| Yang Weihua,Zuo Wenbin,Pan Hui,He Yanyan,Liu Shudong,Xu Lijun,Wang juanjuan,Li Lianrui.Cloning and Sequencing of the Interleukin 8 of Xinjiang Duolang-sheep and Analysis of its Mutant Nucleotide[J].Journal of Tarim University of Agricultural Reclamation,2011,23(1):1-8. | |
| Authors: | Yang Weihua Zuo Wenbin Pan Hui He Yanyan Liu Shudong Xu Lijun Wang juanjuan Li Lianrui |
| Institution: | 1 College of Animal Science,Tarim University,Alar,Xinjiang 843300)(2 College of Life Science,Tarim University,Alar,Xinjiang 843300)(3 Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production and Construction Group,Alar,Xinjiang 843300) |
| Abstract: | The target gene was got by series things such as draw-blood from duolang sheep,isolation of the peripheral blood lymphocytes,isolation of the total mRNA,the two-step RTPCR.The sequence was proved to be the interleukin 8 of duolang sheep after the identification by the electrophoresis,plasmid digestion by restriction enzymes and pairwise alignment of sequences.Also,a SNP(Single Nucleotide Polymorphism) was discovered in the target sequence and proved to be special by databases searching and multiple alignment based on the sequence,and its possible effects on the protein translation was also analyzed. |
| Keywords: | SNP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |