儿茶素富集和EGCG单体纯化新工艺研究

EGCG是绿茶中提取分离出的生物活性物质，是一类具有开发前景的天然产物，将绿茶提取物或茶多酚先经乙酸乙酯，氯仿两种试剂多次富集和脱除咖啡因和色素，得儿茶素总含量不低于95％的茶多酚，再经Sephadex LH-20柱层析纯化，得EGCG单体，用ESI－MS和HPLC等方法鉴定和验证了其化学结构，其纯度均大于96．7％，得率为23．86％，回收率为71．25％。

Study on the new technology of catechins enrichment and EGCG purification

EGCG is a biologic active substance extracted from green tea, and has large exploitation foreground of natural product. The high Purification catechins was enriched at least 5 times by A and B reagents to obtain and taken off caffeine and pigments, whose total content was determined to be above 95% by HPLC. The catechins complex obtained by the above methods was separated and purified by Sephadex LH-20 Column Chromatography to obtain EGCG monomer, whose Purification was over 96%,yield and recycling rate were 23.86% and 71.25% respectively. The molecular structure of EGCG was further confirmed by MS methods.