日本晚樱同源异型基因PrseAP3的克隆及其在单瓣与重瓣花中的表达分析

用同源克隆的方法和3′RACE技术,从日本晚樱(Prunus lannesiana)中分离得到了1个AP3同源基因PrseAP3的cDNA全长,GenBank登录号为JF710371。其cDNA全长977bp,包括1个编码235个氨基酸共708bp的开放阅读框。序列比对和分子系统发生分析表明,PrseAP3是拟南芥的AP3同源基因,其蛋白质的C末端具有2个保守基元:PI-derived模体和paleoAP3模体,属B类转录因子中paleoAP3进化系。半定量RT-PCR分析表明,PrseAP3主要在单瓣日本晚樱品种大岛的萼片、花瓣和雄蕊中表达;在重瓣品种一叶的花瓣、雄蕊和叶化心皮中表达。其表达组织特异性在2个品种间表现出明显的差异,并与拟南芥的AP3基因有一定的差别。

Cloning and expressing analysis of a floral homeotic gene PrseAP3 from Prunus lannesiana

To find genes involved in the floral organogenesis of Prunus lannesiana,cDNAs for one MADS-box genes,PrseAP3 with GenBank accession No.JF710371,was cloned using homologous cloning and 3′-RACE method based on the sequence database of NCBI.The full length of PrseAP3 cDNA is 977 bp,containing an open reading frame(ORF) of 708 bp and coding for a polypeptide of 235 amino acid residues.Sequence comparison and phylogenetic analysis revealed that PrseAP3 was highly homologous to protein AP3 in Arabidopsis and belonged to the paleoAP3 clade of class B.However,semiquantitative RT-PCR analysis showed that PrseAP3 was strongly expressed in petal and stamen of single-flowered P.lannesiana cultivar ’Makino’,and in petal,stamen and leaf-like carpel of double-flowered cultivar ’Hisakura’,obviously different from the expression pattern of AP3 in Arabidopsis.