| 香蕉枯萎菌原生质体形成与再生条件 |
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| 引用本文： | 张欣,谢艺贤,刘志昕,蒲金基,漆艳香,张辉强. 香蕉枯萎菌原生质体形成与再生条件[J]. 热带作物学报, 2007, 28(1): 72-75 |
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| 作者姓名： | 张欣 谢艺贤 刘志昕 蒲金基 漆艳香 张辉强 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所,海南,儋州,571737;热带作物生物技术国家重点实验室,海口,571101;中国热带农业科学院环境与植物保护研究所,海南,儋州,571737;中国热带农业科学院热带生物技术研究所,海口,571101;热带作物生物技术国家重点实验室,海口,571101 |
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| 基金项目： | 中国热带农业科学院科研基金 |
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| 摘 要： | 通过对菌丝菌龄、酶液浓度、酶解时间、酶解温度、渗透压稳定剂的种类和浓度等因素的实验研究,得到了一种制备香蕉枯萎菌(Fusarium oxysporum f.sp.cubense,FOC)原生质体的有效方法,并在固体再生培养基上实现了原生质体的再生,再生率为22.5%。
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| 关 键 词： | 香蕉枯萎菌 原生质体 形成 再生 |
| 修稿时间： | 2005-10-112006-05-12 |
Protoplast Occurrence and Regeneration of Fusarium oxysporum f. sp. cubense |
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| Zhang Xin,Xie Yixian,Liu Zhixin,Pu Jinji,Qi Yanxiang,Zhang Huiqiang. Protoplast Occurrence and Regeneration of Fusarium oxysporum f. sp. cubense[J]. Chinese Journal of Tropical Crops, 2007, 28(1): 72-75 |
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| Authors: | Zhang Xin Xie Yixian Liu Zhixin Pu Jinji Qi Yanxiang Zhang Huiqiang |
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| Affiliation: | 1 Environment and Plant Proteetion Institute, CATAS Danzhou Hainan 571737; 2 Institute of Tropical Bioseienee and Bioteehnology, CATAS Haikou Hainan 571101 ;3 State Key Bioteehnology Laboratory for Tropieal Crops Haikou Hainan 571101 |
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| Abstract: | The effects of some factors on the occurrence and regeneration of protoplasts of Fusarium oxysporum f.sp. cubense were studied. These factors include the appropriate hypha time for isolating protoplasts, the concentrations of enzyme, digesting times and temperatures, and osmotic pressure stabilizers. The purified protoplasts were regenerated on potato dextrose agar medium containing 0.8 mol/L sucrose, and the regeneration rate was 22.5 %. |
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| Keywords: | Fusarium oxysporum f.sp. cubense protoplast occurrence regeneration |
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