|
|||||
|
|
| 高羊茅EST-SSR标记的开发及品种鉴定 | |
| 引用本文： | 段乃彬,王建成,于天祥,孔素萍,李群,张文兰,戴双.高羊茅EST-SSR标记的开发及品种鉴定[J].山东农业科学,2010(10):7-10. |
| 作者姓名： | 段乃彬 王建成 于天祥 孔素萍 李群 张文兰 戴双 |
| 作者单位： | 1. 山东省农业科学院作物研究所,山东,济南,250100 2. 嘉兴检验检疫局,浙江,嘉兴,314001 3. 山东省农业科学院蔬菜研究所,山东,济南,250100 |
| 基金项目： | 嘉兴市科技计划项目“嘉兴市进口草坪草种——高羊茅品种鉴定”(2007AY2004)资助 |
| 摘 要： | 利用EST-SSR分子标记对11份高羊茅（Festuca arundinacea）材料进行了品种鉴定研究。在优化DNA提取方法、扩增体系的基础上对50对EST-SSR引物进行筛选,结果表明：有42对引物可以在高羊茅上得到有效扩增,对11份高羊茅品种的鉴定结果表明,其中12对引物具有良好的多态性,占28.6%;共检测到86个等位基因,平均每个位点的等位基因数为4.5个,变幅为3-12。从谱带类型上看：S3和S44引物扩增的谱带类型最少（2种）,多态性百分率最低,为18.2%;S11引物扩增的谱带类型最多（9种）,多态性百分率最高,为81.8%;共扩增到多态性谱带65种,平均多态百分率为59.1%。 |
| 关 键 词： | 高羊茅 品种鉴定 EST-SSR |
Development of EST-SSR Markers for Cultivar Identification of Festuca arundinacea |
|
| DUAN Nai-bin,WANG Jian-cheng,YU Tian-xiang,KONG Su-ping,LI Qun,ZHANG Wen-lan,DAI Shuang.Development of EST-SSR Markers for Cultivar Identification of Festuca arundinacea[J].Shandong Agricultural Sciences,2010(10):7-10. | |
| Authors: | DUAN Nai-bin WANG Jian-cheng YU Tian-xiang KONG Su-ping LI Qun ZHANG Wen-lan DAI Shuang |
| Institution: | DUAN Nai-bin1,WANG Jian-cheng1,YU Tian-xiang2,KONG Su-ping3,LI Qun1,ZHANG Wen-lan1,DAI Shuang1(1.Crop Research Institute,Sh,ong Academy of Agricultural Sciences,Jinan 250100,China,2.Entry & Exit Inspection , Quarantine Bureau of Jiaxing,Jiaxing 314001,3.Vegetable Research Institute,China) |
| Abstract: | In this study,EST-SSR markers were developed for identification of 11 Festuca arundinacea cultivars at the molecular level.Fifty pairs of EST-SSR primers were screened based on the optimization of DNA extracting method and amplification system.The results showed that 42 pairs of EST-SSR primers had effective amplification,and twelve pairs of them had better polymorphism,which occupied 28.6%.A total of 86 alleles were detected with the mean of 4.5 alleles ranging from 3 to 12.Sixty-five types of polymorphic ... |
| Keywords: | Festuca arundinacea Cultivar identification EST-SSR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |