| 条斑星鲽染色体带型研究 |
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| 引用本文： | 王妍妍,柳学周,徐永江,刘新富. 条斑星鲽染色体带型研究[J]. 渔业科学进展, 2011, 32(4): 6-13 |
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| 作者姓名： | 王妍妍 柳学周 徐永江 刘新富 |
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| 作者单位： | 1. 农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所
2. 青岛市海水鱼类种子工程与生物技术重点实验室,266071 |
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| 基金项目： | 国家鲆鲽类产业技术体系建设项目(CARS-50); 国家“863”计划项目(2006AA10A414;2010AA10A402); 公益性农业行业专项项目(ny-hyzx07-046) |
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| 摘 要： | 开展了条斑星鲽染色体C-带、Ag-NORs带及G-带的研究。结果发现,条斑星鲽染色体显示较为复杂的C-带,有4对染色体整体呈阳性深染,分别为3、13、20、22号染色体;3对具居间C-带,分别为1、4、8号染色体;9号染色体整体显示阴性,其余有大小不一的着丝粒C-带。计算其异染色质含量为54.91%。银染分裂相中Ag-NORs出现的数目以两个的频率最高,达87.5%,与此相一致,在间期核中通过银染表现出两个核仁的间期核数目的频率也最高,故确定条斑星鲽染色体具有1对Ag-NORs,位于亚中部着丝点染色体的短臂上,为端部Ag-NOR。Ag-NORs的数目为1~3个,以出现两个Ag-NOR的频率最高,达87.5%,与此相同的是,在间期核中,通过银染表现出两个核仁的间期核数目的频率也最高。G-带研究显示,条斑星鲽有37条深染带,22条浅染带。
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| 关 键 词： | 条斑星鲽 C-带 Ag-NORs带 G-带 |
| 收稿时间： | 2010-07-23 |
| 修稿时间： | 2011-02-23 |
Study on chromosome banding patterns of Verasper moseri |
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| WANG Yan-yan,LIU Xue-zhou,XU Yong-jiang,LIU Xin-fu. Study on chromosome banding patterns of Verasper moseri[J]. Progress in Fishery Sciences, 2011, 32(4): 6-13 |
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| Authors: | WANG Yan-yan LIU Xue-zhou XU Yong-jiang LIU Xin-fu |
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| Affiliation: | WANG Yan-yan LIU Xue-zhou XU Yong-jiang LIU Xin-fu(Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,266071) |
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| Abstract: | The C-binding,Ag-NORs,and G-banding patterns of the metaphase chromosomes of barfin flounder Verasper moseri were investigated in this study.The results showed that the interstitial regions of most chromosomes exhibited positive C-bands,in which the whole 3rd,13th,20th,and 22nd pair of chromosomes showed totally positive C-band,whilst the 1st,4th and 8th pair of chromosomes only showed positive C-bands in the middle region,the rest with centromeric positive C-bands.However,the 9th pair of chromosomes showed... |
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| Keywords: | Verasper moseri C-binding Ag-NORs G-banding |
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