| 鲐鱼鱼精中5_脱氧核苷酸的分离工艺 |
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| 引用本文： | 吴汉民.鲐鱼鱼精中5_脱氧核苷酸的分离工艺[J].水产学报,2000,24(3):275-279. |
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| 作者姓名： | 吴汉民 |
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| 作者单位： | 吴汉民（宁波大学食品科学研究所,浙江宁波 3l5211）;张芝芬（宁波大学食品科学研究所,浙江宁波 3l5211）;娄永江（宁波大学食品科学研究所,浙江宁波 3l5211）;韩素珍（宁波大学食品科学研究所,浙江宁波 3l5211） |
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| 基金项目： | 农业部渔业局资助项目(鲐鱼鱼精中5＇-脱氧核苷酸的分离工艺研究) |
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| 摘 要： | 论述了用桔青霉PenicilliumcitrinumM71菌株,经液体培养制得的5＇-磷酸二酯酶降解鲐鱼鱼精DNA成5＇-脱氧核苷酸的分离工艺.分离采用201×8阴离子交换树脂,具体条件为柱床高l05mm,柱床直径45mm,样品浓度213mg@mL-1,洗脱流速0.5mL@cm-2@min-1.分离结果表明,采用0.005MHCl+0.04MNaCl作洗脱刺,流速为0.7mL@cm-2@min-1时,四种5'-脱氧核苷酸组分能完全被洗脱下来,且呈一个大峰,同其它成分分开,再先后采用0.0018MHCl、0.0028MHCl、0.036MNaCl(pH6.0)、0.005MHCl+0.02MNaCl作洗脱剂时,则能分别将dCMP、dAMP、TMP、dGMP完全分离.
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| 关 键 词： | 鲐鱼鱼精 脱氧核苷酸 分离工艺 产出率 洗脱剂 |
| 文章编号： | 1000-0615(2000)03-0275-05 |
| 收稿时间： | 2014/4/16 0:00:00 |
| 修稿时间： | 1999年12月16 |
Separating and processing technique of 5..- Deoxymononucleotides from fish spermary of chub mackerel |
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| WU Han min.Separating and processing technique of 5..- Deoxymononucleotides from fish spermary of chub mackerel[J].Journal of Fisheries of China,2000,24(3):275-279. |
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| Authors: | WU Han min |
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| Institution: | Food Science Institute, Ningbo University,Ningbo 315211,China |
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| Abstract: | The process of digesting DNA - Na obtained from f ish spermary of chub mackerel into 5..-
Deoxymononucleotides by using 5..- phosphodiesterase f rom Penicillium citrinum, M71 was studied. The 201 .. 8
anion exchange resin was used in the separation. The conditions for setting samples are height of column bed
105mm, diameter of column bed 45mm, sample concentration 213mg..mL- 1 and eluting flow..rate 0. 5mL..cm- 2
..min
- 1
. The results of the separation indicated 5..- deoxymononucleotides components could be fully eluted as a
whole peak by the elut ing agent, 0. 005 M HCl and 0. 04M NaCl. And the use of a few modified eluting agent s,
i. e. 0. 001 8M HCl, 0. 002 8M HCl, 0. 036M NaCl( pH6. 0) and 0. 005M HCl+ 0. 02M NaCl could bring about
perfect and respective elution of dCMP, dAMP, TMP and dGMP. |
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| Keywords: | chub mackerel fish spermary deoxymononucleotide separating |
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