| 穿山薯蓣不定芽的诱导与植株再生 |
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| 引用本文： | 李际红,谢会成,许建农,韩晓娇,孙仲序.穿山薯蓣不定芽的诱导与植株再生[J].山东农业大学学报(自然科学版),2005,36(1):82-85,92. |
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| 作者姓名： | 李际红 谢会成 许建农 韩晓娇 孙仲序 |
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| 作者单位： | 1. 山东农业大学科技学院,山东,泰安,271000
2. 泰安中医药开发研究中心,泰安,27000 |
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| 摘 要： | 以穿山薯蓣带腋芽茎段为外植体进行离体培养，通过正交试验筛选出最佳的诱导和增殖培养基，利用单因子试验筛选出最佳生根培养基。结果表明，培养基MS BA2．0mg／L NAA0．5mg／L为不定芽诱导的最佳培养基，诱导率为100％；培养基MS BA2．0mg／L NAA0．1mg／L最有利于植株再生，增殖倍数达4．9；培养基1／2MS NAA0．4mg／L对穿龙薯蓣试管苗生根培养较适合，生根率达86．7％；在塑料拱棚中利用蛭石：泥炭土：珍珠岩=3：3：1为移栽基质，移栽成活率可达85％以上。
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| 关 键 词： | 植株再生 带腋芽茎段 增殖培养基 生根培养基 单因子试验 最佳培养基 不定芽诱导 移栽成活率 离体培养 正交试验 增殖倍数 穿龙薯蓣 移栽基质 塑料拱棚 外植体 诱导率 试管苗 生根率 珍珠岩 泥炭土 筛选 |
| 文章编号： | 1000-2324(2005)01-0082-04 |
ADVENTITIOUS BUD INDUCTION AND PLANTLET REGENERATION OF DIOSCOREA NIPPONICA |
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| LI Ji-hong,XIE Hui-cheng,XU Jian-nong,HAN Xiao-jiao,SUN Zhong-Xu.ADVENTITIOUS BUD INDUCTION AND PLANTLET REGENERATION OF DIOSCOREA NIPPONICA[J].Journal of Shandong Agricultural University,2005,36(1):82-85,92. |
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| Authors: | LI Ji-hong XIE Hui-cheng XU Jian-nong HAN Xiao-jiao SUN Zhong-Xu |
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| Institution: | LI Ji-hong1,XIE Hui-cheng1,XU Jian-nong2,HAN Xiao-jiao1,SUN Zhong-xu* |
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| Abstract: | The adventitious bud of Discorea nipponica as explantswere cultured in vitro.The most suitable media for induction and multiplication were selected respectively through orthogonal experiments.The most suitable medium for root generation was also selected through unifactor experiment. The results showed that the MS+BA2.0mg/L+NAA0.5mg/L medium was most suitable for the induction of adventitious bud with the induction rate of 100%.The MS+BA2.0mg/L+NAA0.1mg/L medium was most suitable for plantlet regeneration with multiplication rate of 4.9.The 1/2MS+NAA0.4mg/L medium was most suitable for root generation with rooting rate of 86.7%. In the plastic arch shed, the seedlings were transplanted on substrate composing of vermiculite, turf soil and perlitein proportion of 3:3:1,and the survive rate of transplant seedlings was more than 85%. |
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| Keywords: | Discorea nipponic makino adventitious bud induction plantlet regeneration |
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