5种笛鲷属鱼类的遗传多样性及分子标记

运用RAPD及SSR技术对笛鲷属的画眉笛鲷(Lutjanus vitta)、金带笛鲷 (L. fulvus)、金焰笛鲷 (L. fulviflamma)、千年笛鲷(L. sebae)和星点笛鲷(L. stellatus)等5种鱼的遗传多样性及其分子标记进行了研究。RAPD研究结果表明, 5种鱼的平均多态性位点比率(P)依次为89.30%、86.70%、92.11%、86.47%和86.00%; 种内两个体间平均遗传距离(D)分别为0.3431，0.2130，0.3121, 0.1825和0.1775；平均遗传多样性指数(Hi)分别为0.1634、0.1095、0.1353、 0.1022和0.1024。引物OPA8和OPP10的扩增产物中得到9个RAPD标记，分别为OPA8-413bp、OPA8-140bp、OPP10-418bp、OPA8-697bp、OPP10-526bp、OPA8-361bp、OPP10-449bp、OPA8-311bp和OPP10-599b，可鉴别5种鱼。SSR研究结果显示, 5种鱼的有效等位基因数依次为1.9610、3.3793、3.2957、1.7893和3.6591；群体的杂合度(H)分别为0.332、0.462、0.593、0.367和0.676；多态信息含量(PIC)分别为0.302、0.438、0.554、0.332和0.641。 在11个微卫星位点上得到6个微卫星标记，分别为13 Prs229-115 bp、4 Lca43-212 bp、4 Lca43-240 bp、13 Prs229-288 bp、19 Prs275-156bp和7Lca91-118 bp可用于鉴别5种鱼。

Genetic Diversity and Molecular Markers of 5 Species of Snappers

In order to protect and develop the valuable fishes of snappers, genetic diversity and molecular markers of 5 species of snappers, Lutjanus vitta, L. fulvus, L. fulviflamma, L. sebae and L. stellatus,were detected using RAPD and SSR technique. The amplified products of genomic DNA in the 5 species of snappers by RAPD technique indicated that the polymorphic loci ratio (P ) were 89.30%,86.70%,92.11%,86.47% and 86.00%, the mean genetic distances of intra-species (D) were 0.3431,0.2130,0.3121, 0.1825 and 0.1775, and the genetic diversity indexes of intra-species (Hi ) were 0.1634,0.1095,0.1353, 0.1022 and 0.1024 respectively. Nine molecular markers, OPA8-413bp, OPA8-140bp, OPP10-418bp, OPA8-697bp, OPP10-526bp, OPA8-361bp, OPP10-449bp, OPA8-311bp and OPP10-599b , which could be used for identifying 5 species of snappers were found in the amplified products by primer of OPA8 and OPP10. The amplified products of genomic DNA in 5 species of snappers by SSR technique showed that effective numbers of allelic was 1.9610,3.3793,3.2957,1.7893 and 3.6591 respectively, average heterozygosities were 0.332,0.462,0.593,0.367 and 0.676, polymorphism information contents (PIC) were 0.302,0.438,0.554,0.332 and 0.641 respectively. Six molecular markers, 13 Prs229-115 bp, 4 Lca43-212 bp, 4 Lca43-240 bp, 13 Prs229-288 bp, 19 Prs275-156bp and 7Lca91-118 bp, which could be used for identifying 5 species of snappers were found in 11 loci of micrrosatellite.