Rapid determination of fumonisin B1 in food samples by enzyme-linked immunosorbent assay and colloidal gold immunoassay |
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| Authors: | Wang Shuo Quan Ying Lee Nanju Kennedy Ivan R |
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| Institution: | Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnolgy, Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China. elisasw2002@yahoo.com.cn |
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| Abstract: | A rapid enzyme-linked immunosorbent assay (ELISA) test (microwell plate) and a membrane-based colloidal gold immunoassay in flow-through and lateral-flow formats for the rapid detection of fumonisin B1 (FB1) were developed. The rapid microwell assay can be completed within 20 min with the detection limit of 0.5 +/- 0.2 microg/L. Membrane-based colloidal gold immunoassays had a visual detection limit of 1.0 microg/L for FB1 with the detection time of <10 min. Matrix interference was eliminated by 15-fold dilutions of methanol extracts with buffer. These immunoassays can be used as quantitative or qualitative tools for the rapid detection of FB1 residues in 10-20 min on-site. |
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