Incompatibility on brassica stigmas is overcome by treating pollen with cycloheximide

Pollen of Brassica exhibited strong self-incompatibility. It did not germinate on, adhere to, or extend into the stigmatic tissues of the female parent plant. In contrast, pollen that had been treated with cycloheximide germinated on and penetrated into self-stigmas to the same degree as untreated pollen placed on crossstigmas (compatible). Germ tubes from cycloheximide-treated pollen did not continue growth through stylar tissues and therefore were unable to effect fertilization.