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| 湿地松不定芽增殖培养试验 | |
| 引用本文： | 徐田青,吴丽君,赖钟雄. 湿地松不定芽增殖培养试验[J]. 福建林业科技, 2009, 36(3). DOI: 10.3969/j.issn.1002-7351.2009.03.014 |
| 作者姓名： | 徐田青 吴丽君 赖钟雄 |
| 作者单位： | 1. 福建省林业科学研究院,福建福州350012;福建农林大学园艺植物生物工程研究所,福建福州350002 2. 福建省林业科学研究院,福建福州,350012 3. 福建农林大学园艺植物生物工程研究所,福建福州,350002 |
| 摘 要： | 以湿地松无菌苗带子叶顶芽为外植体,研究了基本培养基、生长调节剂、有机添加物、细胞分裂素等对湿地松不定芽增殖的影响。结果表明:DP+6-BA 4.0 mg.L-1+NAA 0.05 mg.L-1为湿地松不定芽增殖的最佳培养基;培养基附加10 g.L-1肌醇或体积分数为30%的椰子汁对不定芽增殖均有一定的增效作用;培养基中隔代添加6-BA对芽苗伸长生长有利,但对不定芽增殖作用效果不显著。 |
| 关 键 词： | 湿地松 组织培养 不定芽 增殖 |
Proliferation Culture Experiment of Adventitious Bud of Pinus elliottii |
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| XU Tian-qing,WU Li-jun,LAI Zhong-xiong. Proliferation Culture Experiment of Adventitious Bud of Pinus elliottii[J]. Journal of Fujian Forestry Science and Technology, 2009, 36(3). DOI: 10.3969/j.issn.1002-7351.2009.03.014 | |
| Authors: | XU Tian-qing WU Li-jun LAI Zhong-xiong |
| Affiliation: | XU Tian-qing1,2,WU Li-jun1,LAI Zhong-xiong2(1.Fujian Academy of Forestry,Fuzhou 350012,Fujian,China,2.Instiute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
| Abstract: | Pinus elliottii aseptic seedling with the seminal leaf and apical buds were applied as explants.The influences of basic culture medium,growth regulator,organics adding substance,and cytokinin etc.on proliferation of P.elliottii adventitious buds were studied.The results showed that DP+6-BA 4.0 mg·L-1+NAA 0.05 mg·L-1 was the best medium for proliferation of P.elliottii adventitious buds,the culture medium with additions of 10 g·L-1 of inositol and volume fraction of 30% of coconut milk had a certain synergized action for adventitious buds proliferation.Adding 6-BA in septal generation was good for the growth of adventitious buds,but degraded the buds proliferation. |
| Keywords: | Pinus elliottii tissue culture adventitious bud proliferation |
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