Application of virus-induced gene silencing for identification of FHB resistant genes |
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Authors: | Yan-hui FAN Bing-qian HOU Pei-sen SU Hong-yan WU Gui-ping WANG Ling-rang KONG Xin MA Hong-wei WANG |
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Affiliation: | 1. State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai''an 271018, P.R.China;2. Shandong Agricultural University Fertilizer Science Technology Co., Ltd., Feicheng 271600, P.R.China |
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Abstract: | Virus-induced gene silencing(VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus(BSMV)-based VIGS system to evaluate Fusarium head blight(FHB) resistance were explored in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different Fusarium graminearum strains were tested and SF06-1 strain was selected for FHB resistance evaluation. Using this system, Ta AOC, Ta AOS, and Ta OPR3 involved in jasmonic acid(JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing Ta AOS in wheat by stable transgenic plants. |
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Keywords: | Correspondence WANG Hong-wei, Tel: +86-538-8249278, Fax: +86-538-8242226 Triticeae Fusarium head blight (FHB) BSMV-VIGS RNAi |
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