Identification and developmental expression of putative gene encoding juvenile hormone esterase(CpJHE-like) in codling moth,Cydia pomonella(L.) |
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Authors: | Cong HUANG Qiang WU Chun-yan JIANG Long-sheng XING Guo-liang SHI Bin ZHANG Wan-qiang QIAN You-zhi LI Yu XI Nian-wan YANG Fang-hao WAN |
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Affiliation: | 1. College of Plant Protection, Hunan Agricultural University, Changsha 410128, P.R.China;2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;3. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China;4. College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, P.R.China |
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Abstract: | Juvenile hormone esterase(JHE) is a key enzyme for insects, playing an important role in the regulation of insect growth, development, diapause and reproduction. We identified a complete putative JHE of Cydia pomonella(CpJHE-like) which is comprised of a 1 761 bp coding sequence(CDS) encoding 587 amino acid residues from the transcriptome data. The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE(AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE(AaJHE). CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE(MsJHE). Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae. The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva, increased about 300-fold compared to the basal level. Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body. This study indicates that the CpJHE-like is the functional JHE, which may play vital roles in the development and reproduction of C. pomonella. |
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Keywords: | Correspondence HUANG Cong, Mobile: +86-18040634335 juvenile hormone esterase CpJHE-like codling moth |
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