| 几种瓜类枯萎病菌专化型的AFLP分析 |
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| 引用本文： | 段会军,姬惜珠,张彩英,李兴红,郭小敏,马峙英.几种瓜类枯萎病菌专化型的AFLP分析[J].河北农业大学学报,2005,28(5):71-74. |
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| 作者姓名： | 段会军 姬惜珠 张彩英 李兴红 郭小敏 马峙英 |
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| 作者单位： | 1. 河北农业大学,河北,保定071001
2. 北京市农林科学院,北京,100089 |
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| 基金项目： | 河北省自然科学基金资助项目（303188）,河北省教育厅科研基金资助项目（2002141）. |
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| 摘 要： | 利用AFLP分子标记技术对西瓜、冬瓜、黄瓜和甜瓜4个不同专化型的10个菌株进行了分析,结果表明10对引物对供试菌株扩增出550条带,其中多态性带175条,占总带数的31.8%。对供试菌株的AFLP指纹图谱聚类分析,可将其划分为FOGI、FOGII、FOGIII和FOGIV 4个类群(FOGs),分别为西瓜专化型菌株、冬瓜专化型菌株、黄瓜专化型菌株和甜瓜专化型菌株,聚类结果与菌株的专化型间存在明显的对应关系。
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| 关 键 词： | 枯萎病菌 专化型 AFLP分析 |
| 文章编号： | 1000-1573（2005）05-0071-04 |
| 收稿时间： | 2004-01-10 |
| 修稿时间： | 2004-01-10 |
Analysis on the formae speciales of Fusarium oxysporum causing wilts of several cucurbits by AFLP |
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| DUAN Hui-jun,JI Xi-zhu,ZHANG Cai-ying,LI Xing-hong,GUO Xiao-min,MA Zhi-ying.Analysis on the formae speciales of Fusarium oxysporum causing wilts of several cucurbits by AFLP[J].Journal of Agricultural University of Hebei,2005,28(5):71-74. |
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| Authors: | DUAN Hui-jun JI Xi-zhu ZHANG Cai-ying LI Xing-hong GUO Xiao-min MA Zhi-ying |
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| Institution: | 1. Agricultural University of Hebei, Baocling 071001, China; 2. Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China |
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| Abstract: | Ten isolates of 4 gourd formae specialies which were Fusarium oxysporum f.sp.niveum,Fusarium oxysporum f.sp.benincasae,Fusarium oxysporum f.sp.cucumerinum and Fusarium oxysporum f.sp.melonis,were analyzed.The results showd that a total of the 550 AFLP bands were amplified using 10 pairs of primers,and 175 of the 550 bands were polymorphic,31.8% of them were polymorphisms.When all of polymorphic bands were analyzed by cluster analysis the 10 isolates were classified into 4 groups.There exist isolates of 4 different gourd forma specialies in FOGI,FOGII,FOGIII and FOGIV,respectively.There was evidently correspondent relationship between the cluster groups and formae specialies. |
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| Keywords: | Fusarium wilt formae specialis AFLP analysis |
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