| 烤烟薄细胞层培养一次成苗与快繁研究 |
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| 引用本文： | 蒲文宣,杨亿军,孙在军,张新要,宋卫武,黄平俊.烤烟薄细胞层培养一次成苗与快繁研究[J].中国农学通报,2010,26(6):51-53. |
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| 作者姓名： | 蒲文宣 杨亿军 孙在军 张新要 宋卫武 黄平俊 |
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| 作者单位： | 湖南中烟工业有限责任公司技术中心,长沙,410007 |
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| 基金项目： | 湖南中烟工业有限责任公司科技委项目"利用花粉管通道导入野生烟草DNA创造烟草新种质" |
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| 摘 要： | 烤烟（Nicotiana tabacum L）嫩茎3～15层表皮和亚表皮薄细胞层在附加有适当激素配比的MS培养基上可一次培养成苗。薄细胞层一次培养成苗的适宜培养基为：MS+NAA0.1～0.3 mg/l +IBA0.1 mg/l +6-BA1.5～2.5 mg/l+3%蔗糖；BA浓度控制在2.5mg/l以下有利于培育壮苗。适宜的生根培养基为：1/2MS+2%蔗糖+NAA0.1～0.2 mg/l。
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| 关 键 词： | 肉仔鸡 肉仔鸡 大豆磷脂 大豆活性肽 生产性能 胴体品质 |
| 收稿时间： | 2009-09-14 |
| 修稿时间： | 2009-12-16 |
One-step Shoot Formation and Micro Propagation in the Thin Cell-layer Cultures of Flue-cured Tobacco |
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| Pu Wenxuan,Yang Yijun,Sun Zaijun,Zhang Xinyao,Song Waiwu,Huang Pingjun.One-step Shoot Formation and Micro Propagation in the Thin Cell-layer Cultures of Flue-cured Tobacco[J].Chinese Agricultural Science Bulletin,2010,26(6):51-53. |
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| Authors: | Pu Wenxuan Yang Yijun Sun Zaijun Zhang Xinyao Song Waiwu Huang Pingjun |
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| Institution: | Pu Wenxuan,Yang Yijun,Sun Zaijun,Zhang Xinyao,Song Waiwu,Huang Pingjun (Research Center,China Tobacco Hunan Industrial Corporation,Changsha 410007) |
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| Abstract: | The epidermal and sub- epidermal thin cell-layer of flue-cured tobacco (Nicotiana tabacum L) tender stem in additional appropriate hormone combinations of the MS culture medium can take one-step shoot formation. MS+NAA 0.1-0.3 mg/L +IBA 0.1 mg/L +6-BA 1.5-2.5 mg/L+3%Sucrose culture medium is suitable for one-step shoot formation in thin cell layer cultures. A BA concentration below 2.5mg /L is conducive to nurturing strong seedling. 1/2MS+2% Sucrose +NAA 0.1-0.2 mg/L culture medium are suitable for root ind... |
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| Keywords: | tobacco hin cell layer organogenesis |
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