水稻花药培养的影响因素研究

为更好地研究水稻单倍体育种和转基因技术，加速水稻育种进程，以10份寒地水稻品种为试验材料，采用单因素试验方法，研究培养基的琼脂浓度、激素配比、马铃薯水提取液附加物添加及培养方式等因素对水稻花药培养愈伤组织诱导率的影响，优化寒地水稻品种花药组织培养条件。结果表明：10个品种花药组织培养，愈伤组织诱导率差异明显；在琼脂浓度为0．75％左右时，愈伤组织的诱导率达到最高；培养基中激素配比为KT（2mg·L^-1）＋IAA（1mg·L^-1）时诱导效果最佳；培养方式研究中，在双态培养方式下花药愈伤组织诱导率最好，其次为液态培养，再次为固态培养；培养基中添加马铃薯水提取液各处理间差异不显著。

Study on Influencing Factors of Rice Anther Tissue Culture

In order to study rice haploid breeding and transgenic technology hetter and to accelerate the process of rice breeding, taking 10 rice varieties in cold region as experimental materials, single factor experiment was a dopt to study the effect of agar concentration,hormone conbination,potatoes water extract,culture method and other factors on callus induction rate of rice anther culture, anther tissue culture conditions of rice varieties in cold region was optimized. The results showed that 10 varieties of anther was cultured and the difference of an ther callus induction rate was obvious. Agar concentration was about 0.75 ~ ,the callus induction rate reached the highest. When the hormone combination was KT （2 rag-I2l ） ＋ IAA（1 rag＂ L1 ）, induction effect was the best. The callus induction rate of double layer＇s medium culture was the best ioliowed by liquid culture, again for solid culture. Potatoes water extract addition showed there was no significant difference in different treat ments.