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Fate of feed plant DNA monitored in water buffalo (Bubalus bubalis) and rabbit (Oryctolagus cuniculus) |
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| Authors: | Raffaella Tudisco Federico Infascelli Monica Isabella Cutrignelli Fulvia Bovera Caterina Morcia Primetta Faccioli Valeria Terzi |
| Institution: | aDipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Sez. B. Ferrara, Universita di Napoli “Federico II”, Via Delpino, 1-80137 Napoli, Italy bIstituto Sperimentale per la Cerealicoltura, C.R.A., Via San Protaso, 302 29017 Fiorenzuola d' Arda (PC), Italy |
| Abstract: | The effect of the digestion process in the gastro-intestinal tract (GIT) of animal models on the fate and integrity of plant DNA has been widely evaluated since DNA availability and integrity is a key factor for hypothetical horizontal gene transfer of recombinant DNA from GM crop-derived feeds to animal and human gut microflora. In this study, plant DNA sequences from high and low copy number genes were monitored in GIT and tissues of buffaloes and rabbits. Using a real-time PCR approach to track plant DNA in animal samples, we demonstrated the persistence of fragmented plant DNA blood and tissues of buffaloes and rabbits raised with conventional feeding. |
| Keywords: | Buffalo Rabbit Plant DNA survival Real-time qPCR |
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