| 靶向PRRSV核衣壳蛋白N基因的siRNA表达载体的构建及鉴定 |
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| 引用本文： | 曹素芳,李明,王岩,刘长斗,朱赞梅,唐桂芬,肖松云.靶向PRRSV核衣壳蛋白N基因的siRNA表达载体的构建及鉴定[J].安徽农业科学,2009,37(28):13490-13491. |
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| 作者姓名： | 曹素芳 李明 王岩 刘长斗 朱赞梅 唐桂芬 肖松云 |
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| 作者单位： | 郑州牧业工程高等专科学校,河南郑州,450011;郑州先锋动物药业有限公司,河南郑州,450032;河南大明药业有限公司,河南郑州,450027 |
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| 基金项目： | 河南省基础与前沿技术研究计划项目，河南省重点科技攻关，河南省高校科技创新人才支持计划 |
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| 摘 要： | 目的]研究靶向PRRSV核衣壳蛋白N基因的siRNA表达载体的构建及鉴定：方法]设计并人工合成靶向PRRSV核蛋白N基因的3个siRNA靶序列，将其插入CMV启动子下游，克隆到真核表达载体pSilencer4．1-CMV中，对该重组表达载体进行酶切鉴定及DNA测序。结果]成功构建了靶向核蛋白基因表达的siRNA干扰重组质粒载体pSilencer—N。结论]该研究为进一步运用RNA干扰技术进行PRRSV防治研究奠定了基础。
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| 关 键 词： | PRRSV 核蛋白N siRNA 表达载体 |
Construction and Identification of siRNA Expression Vector Targeting Nucleocapsid Protein N Gene of PRRSV |
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| CAO Su-fang.Construction and Identification of siRNA Expression Vector Targeting Nucleocapsid Protein N Gene of PRRSV[J].Journal of Anhui Agricultural Sciences,2009,37(28):13490-13491. |
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| Authors: | CAO Su-fang |
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| Institution: | CAO Su-fang(College of Zhengzhou Animal Husb,ry Engineering,Zhengzhou,Henan 450011) |
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| Abstract: | Objective]The aim was to research the construction and identification of siRNA expression vector targeting nucleocapsid protein N gene of PRRSV.Method]According to sequenced nucieocapsid protein N gene sequence of PRRSV,three siRNA oligonucleotides targeting nucleocapsid protein N gene were designed and synthesized,and were inserted into CMV promoter downstream.Oligonucleotides were inserted into pSilencer 4.1- CMV vector,these recombined plasmids were identified by restriction endonuclease and DNA sequen... |
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| Keywords: | PRRSV siRNA |
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