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| GM-CSF与SS融合表达质粒对小鼠淋巴细胞增殖及GH和IGF-1分泌的影响 | |
| 引用本文： | 舒邓群,茆达干,杨利国,吴志敏,程宝.GM-CSF与SS融合表达质粒对小鼠淋巴细胞增殖及GH和IGF-1分泌的影响[J].扬州大学学报(农业与生命科学版),2008,29(1):11-15. |
| 作者姓名： | 舒邓群 茆达干 杨利国 吴志敏 程宝 |
| 作者单位： | 1. 江西农业大学,动物科技学院,江西,南昌,330045 2. 南京农业大学,动物繁育研究所,江苏,南京,210095 3. 华中农业大学,动物科技学院,湖北,武汉,430070 |
| 摘 要： | 以小白鼠为试验对象,分别肌肉注射生理盐水及pcS/2SS、pGM-CSF/SS、pGM-CSF pcS/2SS质粒,每只50 μg,免疫后第3周以相同剂量加强免疫1次,测定不同质粒对小鼠淋巴细胞增殖反应及血浆生长激素(GH)、胰岛素样生长因子-1 (IGF-1)分泌水平,探讨GM-CSF对生长抑素DNA疫苗的免疫增强作用.结果表明: GM-CSF可增强小鼠脾脏淋巴细胞的增殖反应,以pGM-CSF pcS/2SS组的增殖能力最强,显著高于pcS/2SS组和生理盐水组;同时注射2种表达质粒(pGM-CSF pcS/2SS)的GH分泌水平和融合表达质粒(pGM-CSF/SS)的IGF-1分泌水平明显高于生理盐水组和pcS/2SS免疫组.这证明GM-CSF对生长抑素DNA疫苗有一定的免疫增强作用,可促进生长抑素DNA疫苗的免疫反应,提高血浆GH和IGF-1的分泌水平. |
| 关 键 词： | 小鼠 GM-CSF 生长抑素 淋巴细胞增殖 生长激素 胰岛素样生长因子-1 |
| 文章编号： | 1671-4652(2008)01-0011-05 |
| 修稿时间： | 2006年9月12日 |
Lymphocyte proliferation and GH and IGF-1 secretion of mice immunized with a fusion expression plasmid of GM-CSF and somatostatin |
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| SHU Deng-qun,MAO Da-gan,YANG Li-guo,WU Zhi-min,CHENG Bao.Lymphocyte proliferation and GH and IGF-1 secretion of mice immunized with a fusion expression plasmid of GM-CSF and somatostatin[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2008,29(1):11-15. | |
| Authors: | SHU Deng-qun MAO Da-gan YANG Li-guo WU Zhi-min CHENG Bao |
| Institution: | 1.Coll of Anim Sci and Tech;Jiangxi Agric Univ;Nanchang 330045;China;2.Res Inst of Anim Breed and Repr;Nanjing Agric Univ;Nanjing 210095;3.Coll of Anim Sci and Tech;Huazhong Agric Univ;Wuhan 430070;China |
| Abstract: | A total of 40 mice at the age of 22 days was divided into 4 groups to study the immuno-enhancement of genetic adjuvant GM-CSF on somatostatin(SS)DNA vaccine.Ten mice in each group were given two intramuscular injections at interval of 2 weeks with saline solution as control(group 1),pcS/2SS(group 2),pGM-CSF/SS(group 3),and pGM-CSF+pcS/2SS(group 4),respectively.The results showed that lymphocyte proliferation response of pcS/2SS along with pGM-CSF was higher than that of the others,indicating that GM-CSF pos... |
| Keywords: | mice GM-CSF somatostatin lymphocyte proliferation growth hormone insulin growth factor-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |