洋常春藤法呢基焦磷酸合酶基因的克隆与序列分析

以洋常春藤叶片为材料,采用同源RT–PCR方法,克隆获得了洋常春藤法呢基焦磷酸合成酶基因,其c DNA序列大小为1 050 bp,开放阅读框为1 026 bp,推测编码342个氨基酸残基,该序列的核苷酸、氨基酸与五加科植物有同源性;用在线数据软件进行分析,推测该蛋白可能存在于细胞质之中,定位在细胞膜外,FPS蛋白的相对分子质量为39 735.7;该蛋白含有2个天冬氨酸保守结构域,其二级结构主要以α螺旋和无规则卷曲为主;双子叶植物系统进化树分析结果表明,洋常春藤的FPS与刺五加、人参、三七、西洋参、辽东楤木的FPS亲缘关系最近,这一结果符合传统的分类法规则。

Design of an Automatic Tracking of Intelligent Logistics Information Processing System

In order to implement the traceability of logistics goods, real-time monitoring of the vehicle and logistics information collection and processing, this paper designed an intelligent logistics information processing system, which uses ARM micro controller as the hardware core, records the process of supplies packaging, warehousing, transportation etc via RFID technology, transmits materials information to the monitoring platform with GPRS network. And the users can log on to the platform to obtain real-time logistics transportation situation of vehicle, the geographical position, material information. The test results show that the system is stable and reliable in achieving the whole process of logistics tracking, real-time monitoring, information transmission and other functions.