| 杨树生物钟节律基因PtCCA1的克隆及表达模式研究 |
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| 引用本文： | 刘无双,杜明会,陶维康,杨贞,诸葛强.杨树生物钟节律基因PtCCA1的克隆及表达模式研究[J].林业科学研究,2013,26(5):649-654. |
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| 作者姓名： | 刘无双 杜明会 陶维康 杨贞 诸葛强 |
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| 作者单位： | 林木遗传与生物技术省部共建教育部重点实验室,南京林业大学,江苏南京210037 |
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| 基金项目： | 国家自然科学基金项目(30571518) |
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| 摘 要： | 生物钟节律基因CCA1在植物的光周期反应中起着重要的调控作用.利用RT-PCR方法克隆获得了一条包含1902 bp开放阅读框的杨树CCA1基因cDNA序列,其编码633个氨基酸残基,蛋白的分子量约为68.90 kDa,等电点为6.33.分析显示含有1个Myb-like DNA结合域及2个蛋白跨膜结合域.实时荧光定量PCR分析发现,PtCCA1基因主要在杨树叶组织中表达;随着光照时间的变化,该基因在杨树中的表达量呈现白昼不断降低而夜晚逐渐升高的昼夜变化趋势.研究结果为进一步探索PtCCA1基因在调控杨树光周期响应途径中的功能以及杨树光周期敏感机制提供了科学基础.
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| 关 键 词： | 杨树 RtCCA1 生物节律钟 基因表达 |
| 收稿时间： | 2013/5/29 0:00:00 |
Cloning and Expression Analysis of Circadian Clock Gene CCA1 in Populus |
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| LIU Wu-shuang,DU Ming-hui,TAO Wei-kang,YANG Zhen and ZHUGE Qiang.Cloning and Expression Analysis of Circadian Clock Gene CCA1 in Populus[J].Forest Research,2013,26(5):649-654. |
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| Authors: | LIU Wu-shuang DU Ming-hui TAO Wei-kang YANG Zhen and ZHUGE Qiang |
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| Institution: | Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China |
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| Abstract: | CCA1 gene plays an important role in circadian clock sensitivity in Populus. In this study, PtCCA1 was isolated from Populus tomentosa by RT-PCR. It has an open reading frame (ORF) of 1902 bp in length and encodes a protein of 663 amino acid residues, with protein molecular weight of 68.90 kDa and isoelectric point of 6.33. The amino acid sequence contained one Myb-like DNA binding domains and two putative transmembrane spanning regions. The results of fluorescence quantitative RT-PCR showed that the PtCCA1 gene was expressed mainly in leaf tissues. Along with the change of illumination time, the expression amount of PtCCA1 gene decreased during the day but gradually increased during the night. This study provides some references for further study of PtCCA1 function in regulating photoperiod sensitivity and the mechanism of circadian synchronization in poplars. |
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| Keywords: | Populus PtCCA1 circadian clock gene expression |
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