| 牛白细胞黏附缺陷病液相基因芯片检测方法的建立 |
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| 引用本文： | 谢明星,彭小莉,苗春柳,刘棠,陈伟玲,王群力,王景明,周斌华,徐维佳. 牛白细胞黏附缺陷病液相基因芯片检测方法的建立[J]. 中国奶牛, 2013, 0(17): 48-52 |
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| 作者姓名： | 谢明星 彭小莉 苗春柳 刘棠 陈伟玲 王群力 王景明 周斌华 徐维佳 |
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| 作者单位： | 1. 云南出入境检验检疫局,昆明,650228
2. 厦门出入境检验检疫局,厦门,360012 |
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| 基金项目： | 国家质量监督检验检疫总局2008年度科研项目 |
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| 摘 要： | 从福建某奶牛场200头进口荷斯坦牛采血提取牛血液DNA，根据已知牛染色体上CD18编码基因序列383位基由A变为G而引起牛白细胞黏附缺陷症（BLAD）设计特异性野生型和突变型引物，建立液相基因芯片检测方法用于牛白细胞黏附缺陷症（BLAD）的检测，结果检出2头母牛为BLAD携带者，检出率为0．67％，没有发现患病牛。结果证明建立的液相基因芯片检测方法是一种敏感性和特异性很高的筛选奶牛BLAD有害基因的新方法
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| 关 键 词： | 牛白细胞黏附缺陷症（BLAD） 液相基因芯片 检测方法 |
Development of a Liquid Gene Chip Assay for Detecting Bovine Leukocyte Adhesion Deficiency in Cattle |
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| XIE Ming-xingL,PENG Xiao-li,MIAO Chun-liu,LIU Tang,CHEN Wei-ling,WANG Qun-li,WANG Jing-ming,ZHOU Bin-hua,XU Wei-jia,XU Qiu-bei. Development of a Liquid Gene Chip Assay for Detecting Bovine Leukocyte Adhesion Deficiency in Cattle[J]. China Dairy Cattle, 2013, 0(17): 48-52 |
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| Authors: | XIE Ming-xingL PENG Xiao-li MIAO Chun-liu LIU Tang CHEN Wei-ling WANG Qun-li WANG Jing-ming ZHOU Bin-hua XU Wei-jia XU Qiu-bei |
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| Affiliation: | 1. Yunnan Entry-exit Inspection and Quarantine Bureau, Kunming 650228; 2. Xiamen Entry-exit Inspection and Quarantine Bureau, Xiamen 360012) |
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| Abstract: | A liquid gene chip assay based on Luminex xMAP system was established and applied to detect bovine leukocyte adhesion deficiency in Holstein cows. A total of 200 Holstein cows imported from foreign countries were genotyped by liquid gene chip assay. Two cows were identified as BLAD carriers,corresponding to the carrier frequency 0.67%, there was no patient cows. The results showed that the liquid gene chip assay is a new sensitive and specific assay for genotyping BLAD. |
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| Keywords: | BLAD Liquid gene chip assay Detecting method |
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