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RAPD and ISSR molecular markers in <Emphasis Type="Italic">Olea europaea</Emphasis> L.: Genetic variability and molecular cultivar identification
Authors:Paula Martins-Lopes  José Lima-Brito  Sónia Gomes  Julieta Meirinhos  Luís Santos  Henrique Guedes-Pinto
Institution:(1) Department of Genetics and Biotechnology, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal;(2) Department of Oliviculture, National Plant Breeding Station, INIA, P.O. Box 6, 7350-951 Elvas, Portugal
Abstract:Thirty Portuguese and eight foreign olive (Olea europaea L.) cultivars were screened using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) markers. Twenty RAPD primers amplified 301 reproducible bands of which 262 were polymorphic; and 17 ISSR primers amplified 204 bands of which 180 were polymorphic. The percentage of polymorphic bands detected by ISSR and RAPD was similar (88 and 87%, respectively). The genetic variability observed was similar in the Portuguese and foreign olive cultivars. Seven ISSR and 12 RAPD primers were able to distinguish individually all 38 olive cultivars. Twenty specific molecular markers are now available to be converted into Sequence Characterised Amplified Region (SCAR) markers. Relationships among Portuguese and foreign cultivars is discussed.
Keywords:Genetic variability  ISSR  Molecular cultivar identification            Olea europaea L    RAPD
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