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沙芥花粉萌发特性和柱头可授性的研究
引用本文:黄修梅,郝丽珍,胡宁宝,赵清岩,张进文,王六英,田志来.沙芥花粉萌发特性和柱头可授性的研究[J].园艺学报,2008,35(10):1473-1478.
作者姓名:黄修梅  郝丽珍  胡宁宝  赵清岩  张进文  王六英  田志来
作者单位:(内蒙古农业大学农学院,呼和浩特 010019;内蒙古农业大学生态环境学院,呼和浩特 010019)
基金项目:国家自然科学基金,内蒙古科技攻关项目,内蒙古自然科学基金,内蒙古人才工程项目
摘    要: 用培养基发芽法研究沙芥花粉萌发特性;用人工异株授粉法和联苯胺—过氧化氢法测定柱头可授期。结果表明:沙芥花粉萌发的最佳培养基是20%蔗糖+0.001%硼酸;花粉能够萌发的温度范围为15~38 ℃,最适萌发温度为25 ℃;从花蕾充分膨大期至开花后16 h花粉活力较强,开花后48 h花粉基本无活力;花粉储藏的适宜条件为低温(4 ℃),储藏期为7 d。柱头可授期为开花前48 h至开花后96 h,其中开花后24~72 h可授性强。

关 键 词:沙芥  花粉  萌发特性  柱头可授性  温度  培养基
收稿时间:2008-05-12

Pollen Germination Characteristic and Stigma Receptivity of Pugionium cornutum
HUANG Xiu-mei,HAO Li-zhen,HU Ning-bao,ZHAO Qing-yan,ZHANG Jin-wen,WANG Liu-ying,TIAN Zhi-lai.Pollen Germination Characteristic and Stigma Receptivity of Pugionium cornutum[J].Acta Horticulturae Sinica,2008,35(10):1473-1478.
Authors:HUANG Xiu-mei  HAO Li-zhen  HU Ning-bao  ZHAO Qing-yan  ZHANG Jin-wen  WANG Liu-ying  TIAN Zhi-lai
Institution:(College of Agronomy Inner Mongolia Agricultural University, Huhohot 010019,China;College of ecology and environment Inner Mongolia Agricultural University, Huhohot 010019,China)
Abstract:Pollen germination characteristic of Pugionium cornutum (L.)Gaerner was evaluated by germination method on culture medium, its stigma receptivity was estimated by the hand cross-pollination and benzidine-H2O2 method. The results showed the optimal pollen germination of P.cornutum was on the culture medium added 20% sucrose and 0.001% boric acid. The effective temperature of pollen germination was from 15 ℃ to 38 ℃ and the optimal temperature was 25 ℃. The pollen germination rate was very higher from the biggest bud stage to 16 h after blooming, and the pollen viability was disappeared 48 h after blooming. The suitable condition of storing pollen was low temperature(4 ℃), and storing period was 7 d. The stigma of P.cornutum had receptivity from 48 h before blooming to 96 h after blooming,the strong receptivity in 24—72 h after blooming.
Keywords:Pugionium cornutum (L  Gaertner Pugionium cornutum (L  Gaertner  pollen  germination characteristic  stigma receptivity  temperature  medium
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