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| PCR法检疫鉴定番石榴实蝇 | |
| 引用本文： | 余道坚,邓中平,陈志粦,焦懿,章桂明,康林,杨伟东,金显忠. PCR法检疫鉴定番石榴实蝇[J]. 植物检疫, 2004, 18(2): 73-76 |
| 作者姓名： | 余道坚 邓中平 陈志粦 焦懿 章桂明 康林 杨伟东 金显忠 |
| 作者单位： | 1. 深圳出入境检验检疫局,518010;中国科学院上海植物生理生态研究所 2. 中山大学昆虫学研究所,生物防治国家重点实验室 3. 深圳出入境检验检疫局,518010 |
| 基金项目： | 广东省深圳市出入境检验检疫局科研项目 |
| 摘 要： | 利用mtDNA序列,设计出两对特异性引物,应用定性PCR技术检疫鉴定番石榴实蝇.本方法适用于成虫鉴定和未成熟虫态(卵、幼虫、蛹)的快速检疫鉴定. |
| 关 键 词： | 鉴定 番石榴实蝇 |
Method of the polymerase chain reaction for quarantine and identification of Bactrocera correcta |
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| Yu Daojian ,,Deng Zhongping,Chen Zhilin,Jiao Yi,Zhang Guiming,Kang Lin,Yang Weidong,Jin Xiangzhong. Method of the polymerase chain reaction for quarantine and identification of Bactrocera correcta[J]. Plant Quarantine, 2004, 18(2): 73-76 | |
| Authors: | Yu Daojian Deng Zhongping Chen Zhilin Jiao Yi Zhang Guiming Kang Lin Yang Weidong Jin Xiangzhong |
| Affiliation: | Yu Daojian 1,2,Deng Zhongping3,Chen Zhilin1,Jiao Yi1,Zhang Guiming1,Kang Lin1,Yang Weidong1,Jin Xiangzhong1 |
| Abstract: | A PCR-based method of Bactrocera correctus species identification based on nucleotide sequences of the mtDNA has been established.Two specific primes for B.correctus have been designed.It is useful for rapidly inspecting and identifying either the adult of B.correctus or the immature stages such as eggs,larvae and pupae. |
| Keywords: | PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |