| 副结核分枝杆菌hsp65基因的克隆及序列分析 |
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| 引用本文： | 徐凤宇,胡玉庆,曾范利,姜秀云,何昭阳. 副结核分枝杆菌hsp65基因的克隆及序列分析[J]. 吉林农业大学学报, 2009, 31(2) |
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| 作者姓名： | 徐凤宇 胡玉庆 曾范利 姜秀云 何昭阳 |
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| 作者单位： | 吉林农业大学动物科技学院,长春,130118;吉林农业大学生命科学学院,长春,130118 |
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| 基金项目： | 国家自然科学基金，吉林农业大学青年启动基金 |
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| 摘 要： | 以副结核分枝杆菌C-2株基因组DNA为模板,以hsp65基因特异性引物进行PCR扩增,获得了1 626 bp的DNA片段.通过T-A克隆技术,将PCR产物克隆至pGEM-T载体.经鉴定,成功地构建出了重组质粒pGEM-T-hsp65.序列分析结果表明:该序列与GenBank中副结核分枝杆菌K～10株的同源性为99.2%.
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| 关 键 词： | 副结核分枝杆菌 hsp65基因 克隆 序列分析 |
Cloning and Sequence Analysis of Mycobacterium paratuberculosis hsp65 gene |
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| XU Feng-yu,HU Yu-qing,ZENG Fan-li,JIANG Xiu-yun,HE Zhao-yang. Cloning and Sequence Analysis of Mycobacterium paratuberculosis hsp65 gene[J]. Journal of Jilin Agricultural University, 2009, 31(2) |
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| Authors: | XU Feng-yu HU Yu-qing ZENG Fan-li JIANG Xiu-yun HE Zhao-yang |
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| Affiliation: | XU Feng-yu1,HU Yu-qing1,ZENG Fan-li1,JIANG Xiu-yun2,HE Zhaoyang11.College of Animal Science , Technology,Jilin Agricultural University,Changchun 130118,China,2.College of Life Science |
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| Abstract: | The genomic DNA was extracted from Mycobacterium paratuberculosis(MP) strain C-2.The secreted protein hsp65 gene was amplified with a pair of specific primers using polymerase chain reaction(PCR).PCR product was anapproximate 1 626 bp DNA segment.The clone vector pGEM-T-hsp65 was constructed successfully by the PCR product that was cloned into pGEM-T vector using T-A clone technique, and the recombinant clone was identified by using complementation test,plasmid size test,restrictional enzyme assay,plasmid P... |
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| Keywords: | Mycobacterium paratuberculosis hsp65 gene cloning sequence analysis |
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