| 转基因鱼品系的纯化 |
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| 引用本文： | 龙华. 转基因鱼品系的纯化[J]. 南方水产, 2005, 1(5): 60-62 |
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| 作者姓名： | 龙华 |
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| 作者单位： | 中国水产科学研究院长江水产研究所,农业部淡水鱼类种质资源与生物技术重点开放实验室,湖北,荆州,434000 |
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| 基金项目： | 中国水产科学研究院人才培养专项基金项目“GFP标记转基因草鱼在环境水体监测上的应用研究” |
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| 摘 要： | 外源基因(双链DNA)作为外显子可能整合在一条染色体上的不同DNA链上,转基因鱼的第一代为嵌合体。三杂交纯化过程方可获得转基因鱼纯合体:嵌合体与纯系个体两次回交,获得杂合子单一的杂合体;第二代杂合体自交可以获得纯合体。
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| 关 键 词： | 转基因鱼 嵌合体 杂合体 纯合体 |
| 文章编号： | 1673-2227-(2005)05-0060-03 |
| 修稿时间： | 2005-06-23 |
Depuration processes of transgenic fish inbred strain |
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| LONG Hua. Depuration processes of transgenic fish inbred strain[J]. South China Fisheries Science, 2005, 1(5): 60-62 |
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| Authors: | LONG Hua |
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| Abstract: | Foreign gene (double stranded DNA) is maybe integrated in different DNA strand of the same chromosome as an exon. The first generation of transgenic fish is mosaic. The homozygote generation of transgenic fish is maybe obtained by the three-cross depuration process. The heterozygote generation with single heterozygosis is obtained by twice backcross mating between mosaic and pure individual. Finally, the homozygote generation is obtained by self-cross mating of the second heterozygote. |
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| Keywords: | transgenic fish mosaic heterozygote homozygote |
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