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| 不同油松纯林中油松毛虫的遗传多样性及环境因素分析 | |
| 引用本文： | 杜娟,郭红珍,高宝嘉,袁一杨. 不同油松纯林中油松毛虫的遗传多样性及环境因素分析[J]. 河北农业大学学报, 2011, 34(1): 82-86 |
| 作者姓名： | 杜娟 郭红珍 高宝嘉 袁一杨 |
| 作者单位： | 1. 廊坊师范学院,河北,廊坊,065000 2. 河北北方学院,河北,张家口,075000;河北农业大学,河北,保定,071000 3. 河北农业大学,河北,保定,071000;中国科学院动物研究所,北京,100101 |
| 基金项目： | 廊坊师范学院自然科学青年基金; 国家自然科学基金(30771739) |
| 摘 要： | 采用AFLP技术对平泉县的2个油松纯林中油松毛虫进行了遗传多样性和遗传结构的研究。5组引物组合对90个样品DNA进行PCR扩增,并进行变性聚丙烯酰胺凝胶电泳分析,调查了2个油松林群落的环境条件。结果表明,纯林中松毛虫的遗传分化低于环境复杂的林分类型。运用POPGENE软件对2个油松毛虫种群的遗传多样性进行分析,并运用SPSS软件对影响油松毛虫种群的环境因子进行主成分分析。结果表明:2个油松纯林的油松毛虫的多态位点百分率为81.51%,总基因多样度Ht为0.259 6,基因流Nm为6.280 9,不同油松纯林中影响油松毛虫遗传多样性的各种环境因素中树高得分最高(0.473 6),说明2个油松毛虫种群之间基因交流非常强烈,林木生长状况是影响油松毛虫种群遗传多样性的主要原因,油松林的立地条件也有一定影响,物种多度的影响较小。 |
| 关 键 词： | 油松毛虫 AFLP 遗传多样性 环境因子 |
Analysis of environmental factors influencing genetic diversity of Dendrolimus tabulaeformis in different Pinus tabulaeformis pure forests |
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| DU Juan,GUO Hong-zhen,GAO Bao-jia,YUAN Yi-yang. Analysis of environmental factors influencing genetic diversity of Dendrolimus tabulaeformis in different Pinus tabulaeformis pure forests[J]. Journal of Agricultural University of Hebei, 2011, 34(1): 82-86 | |
| Authors: | DU Juan GUO Hong-zhen GAO Bao-jia YUAN Yi-yang |
| Affiliation: | DU Juan1,GUO Hong-zhen1,GAO Bao-jia2,3,YUAN Yi-yang3,4 (1.Life Sciences College,Langfang Teachers College,Langfang 065000,China,2.Hebei North University,Zhangjiakou 075000,3.Agricultural University of Hebei,Baoding 071000,4.Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China) |
| Abstract: | Using amplified fragment length polymorphism(AFLP) technique,genetic diversity and genetic structure of Dendrolimus tabulaeformis populations in 2 Pinus tabulaeformis pure forests from Pingquan County were analyzed.Moreover the environmental conditions of 2 communities were also investigated.DNA extracted from 90 individuals were amplified using 5 pairs primers.Genetic diversity of two Dendrolimus tabulaeformis populations was analyzed by POPGENE software.Finally,the principle component analysis method was ... |
| Keywords: | Dendrolimus tabulaeformis AFLP genetic diversity environmental factors |
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