| H3亚型猪流感病毒分离与鉴定 |
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| 引用本文： | 黄良宗,顾万军,王淑敏,冼琼珍. H3亚型猪流感病毒分离与鉴定[J]. 动物医学进展, 2006, 27(6): 61-63 |
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| 作者姓名： | 黄良宗 顾万军 王淑敏 冼琼珍 |
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| 作者单位： | 佛山科学技术学院动物医学系,广东佛山528231;佛山科学技术学院动物医学系,广东佛山528231;佛山科学技术学院动物医学系,广东佛山528231;佛山科学技术学院动物医学系,广东佛山528231 |
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| 摘 要： | 从东莞和鹤山等地不同猪场采集的40份鼻拭子或病死猪的肺、气管病料中分离到4株有血凝活性的病毒,其中3株病毒与新城疫病毒阳性血清的HI试验为阳性,另外1株病毒与抗猪流感H3亚型猪流感病毒阳性血清的HI试验为阳性;根据猪流感病毒M基因设计引物,扩增出预期的约315 bp片段,表明该病毒为H3亚型猪流感病毒。
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| 关 键 词： | 猪流感病毒 分离 鉴定 血凝抑制试验 逆转录-聚合酶链反应 |
| 文章编号： | 1007-5038（2006）06-0061-03 |
| 收稿时间： | 2006-03-13 |
| 修稿时间： | 2006-03-13 |
Isolation and Identification of H3 Sub-type Swine influenza virus |
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| HUANG Liang-zong,GU Wan-jun,WANG Shu-min,XIAN Qiong-zhen. Isolation and Identification of H3 Sub-type Swine influenza virus[J]. Progress In Veterinary Medicine, 2006, 27(6): 61-63 |
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| Authors: | HUANG Liang-zong GU Wan-jun WANG Shu-min XIAN Qiong-zhen |
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| Affiliation: | Department of Veterinary Medicine ,Foshan University , Foshan,Guangdong , 528231,China |
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| Abstract: | 4 strains virus was isolated from 40 swabs samples of swine collected from Dongguan and Heshan with chicken embryo.The virus strains proved to be have hemagglu-tination activity.The HI test against Newcastle disease virus of 3 strains virus were positive and the other one was negative.And the HI test against H3 sub-type Swine influenza virus was positive.Then a pair of primer was designed according to M gene of Swine influenza virus published in the GenBank.About 315 bp fragment can be amplified by RT-PCR.The results showed that the virus was H3 sub-type Swine influenza virus. |
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| Keywords: | Swine influenza virus isolation identification HI RT-PCR |
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