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刺葡萄酿酒酵母菌株的分离与筛选
引用本文:王瑞琛,伍婧,刘静,吴静,秦丹,胡亚平.刺葡萄酿酒酵母菌株的分离与筛选[J].保鲜与加工,2013,13(3):47-50.
作者姓名:王瑞琛  伍婧  刘静  吴静  秦丹  胡亚平
作者单位:湖南农业大学食品科学技术学院,湖南长沙,410128;湖南农业大学食品科学技术学院,湖南长沙410128;食品科学与生物技术湖南省重点实验室,湖南长沙410128;湖南省发酵食品工程技术研究中心,湖南长沙410128
基金项目:湖南省科技厅重点项目(2011NK2018);长沙市科技局重点项目(k1207090-21);湖南省大学生创新项目(SCX1105)
摘    要:为寻找适合酿造刺葡萄果酒的酵母菌株,从刺葡萄原酒、新鲜刺葡萄以及经初发酵过滤所得的刺葡萄皮渣中分离提取得到90株酿酒酵母,并对经发酵试验初步筛选出的5株酵母进行了耐酒精、耐糖、耐酸和耐二氧化硫的性能测定比较,最终筛选出1株发酵性能优良的菌株(B54)。该菌株具有很高的耐酒精能力,在18%的酒精浓度条件下也能迅速启动发酵,同时在高浓度SO2(250 mg/L)条件下发酵启动最快,并能耐受2.0%浓度的柠檬酸,较其他菌株具有更强的耐酸性,因此可作为刺葡萄酿酒的专用菌株。

关 键 词:刺葡萄  酿酒酵母  分离  性能

The Isolation and Screening of Saccharomyces cerevisiae Strains of Vitis davidii Foex
WANG Rui-chen,WU Jing,LIU Jing,WU Jing,QIN Dan,HU Ya-ping.The Isolation and Screening of Saccharomyces cerevisiae Strains of Vitis davidii Foex[J].Storage & Process,2013,13(3):47-50.
Authors:WANG Rui-chen  WU Jing  LIU Jing  WU Jing  QIN Dan  HU Ya-ping
Institution:1. Food Science and Technology College, Hu'nan Agricultural University, Changsha 410128, China; 2. Key Laboratory of Food Science and Biological Technology in Hu'nan Province, Changsha 410128, China; 3. Fermented Food Engineering and Technology Research Center in Hu'nan Province, Changsha 410128, China)
Abstract:In order to find a suitable yeast strain for brewing Vitis davidii Foex wine, 90 Saccharomyces cerevisiae strains were extracted from unblended wine of Vitis davidii Foex, fresh Vitis davidii Foex and grape residue left after being primarily fermented and filtered. Through preliminary selection of fermentation experiment, we got 5 strains of yeast. And through comparison of performance measurement such as alcohol tolerance, sugar tolerance, acid resistance and sulfur dioxide resistance, we screened one yeast strain(B54) with fermentation performance excellent finally. B54 had the strongest alcohol tolerance capacity, could start fermentation quickly in the 18% alcohol concentration, was the fastest start fermentation yeast under the high concentration of SO2 (250 mg/L), could tolerate 2% citric acid, had stronger acid resistance than other strains. So, it could be used as a special strain of Vitis davidii Foex wine.
Keywords:Vitis davidii Foex  Saccharomyces cerevisiae  extraction  performance
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