Molecular cloning of a cellulose synthase gene PtoCesA1 from Populus tomentosa and its genetic transformation in tobacco |
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Authors: | Li Chun-xiu Zhang Shou-gong Wang Yang-dong Shi Sheng-qing and Qi Li-wang |
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Institution: | (1) Laboratory of Cell Biology, Research Institute of Forestry, Chinese Academy of Forestry, 100091 Beijing, P. R. China |
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Abstract: | A 3 125 bp cellulose synthase gene, PtoCesA1, which has a 98% identity to PtrCesA1 from Populus tremuloides, was cloned from cDNA prepared from secondary xylem of P. tomentosa. Four anti-expression vectors with different fragments of PtoCesA1, named as pBIPF, pBICC1, pBIPR and pBIBR, were constructed. Some traits of transformed tobacco of pBICC1, pBIPR and pBIBR
differed from wild types, such as small leaves, “dwarf” phenotype and thinner xylem and fiber cell walls than wild plants
consistent with a loss of cellulose. It indicated that the growth of transgenic tobacco was restrained by the expression of
anti-PtoCesA1. Transgenic tobacco was obtained and the contents of cellulose and lignin were analyzed as well as the width and length of
fiber cells, and xylem thickness for both transgenic and control plants. Transformed tobacco showed a different phenotype
from control plants and it implied that PtoCesA1 was essential for the cellulose biosynthesis in poplar stems.
Supported by the Hi-Tech Research and Development Program of China (863) (2001AA244060 and 2003AA244020) and National Basic
Research Program of China (973) (J1999016003)] |
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Keywords: | Populus tomentosa cellulose synthase transgenic tabacco PtoCesA1 |
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