寿光鸡胚原始生殖细胞的形态和分布观察

采用连续切片法进行鸡胚胎显微观察，研究寿光鸡原始生殖细胞（PGCs）形态和分布规律。试验结果表明，PGCs在迁移过程中出现3次聚集高峰。第1次是在孵化19～22 h时聚集在生殖新月处；第2次是在孵化48～55 h时聚集在心脏和背主动脉中；第3次是孵化4~5 d时的生殖嵴中。生殖新月区的PGCs呈不规整的圆形或卵圆形，细胞内含有大量的糖原颗粒，血液中PGCs呈规整的圆形。性腺中PGCs呈圆形或卵圆形，有伪足。胞核均呈圆形或椭圆形，偏向细胞边缘。细胞直径为10~22.5 μm，细胞核的大小均为7.5 μm左右。

Observation about the Morphous and Distribution of Primoridial Germ Cells in Shouguang Chicken's Embryo

The morphous and distribution rule of primoridial germ cells (PGCs)in chicken’s embryo was studied. The results showed that there were three aggregation peaks during the migration of the PGCs. When hatched 19～22 hours, most PGCs gathered at the germinal crescent. Then, when hatched 48～55 hours most PGCs migrated at the heart and dorsal aorta. At last, most PGCs ecesised at the ganadal ridge when hatched 4～5 days. The shape of PGCs in the germinal crescent liked round or orbicular ovate, there were large glycogen granule in the cells. PGCs in the blood was round. And PGCs in the gonad were round or orbicular ovate and had spurious. The nucleus was all round or orbicular ovate at one side of the cell. The diameter of the cell was 10~22.5 μm and the nuclear was about 7.5 μm.