| 菜用大豆高效离体再生体系的优化 |
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| 引用本文： | 张古文,丁桔,徐盛春,陆新苗,胡齐赞,龚亚明.菜用大豆高效离体再生体系的优化[J].浙江农业学报,2011,23(1):79. |
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| 作者姓名： | 张古文 丁桔 徐盛春 陆新苗 胡齐赞 龚亚明 |
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| 作者单位： | 1.浙江省农业科学院 蔬菜研究所,浙江 杭州 310021；2 宁海县农技推广总站,浙江 宁海 315600 |
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| 基金项目： | 浙江省科技计划项目(2009C32094); 浙江省农业科学院创新提升工程项目(2008R23Y01D02,2009R23Y01D05) |
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| 摘 要： | 为筛选较易再生的菜用大豆品种,为遗传转化建立良好的再生体系,以‘春丰早’、‘浙农6号’、‘浙农8号’、‘沪宁95-1’、‘辽鲜1号’和‘台湾75’为材料,研究外植体类型、激素配比、接种时间、接种方式以及基因型对再生频率的影响。结果表明：不同外植体类型中,子叶—子叶柄的再生频率最高；B5培养基+10 mg·L-1 TDZ+005 mg·L-1 NAA+50 mg·L-1 AgNO3为最佳培养基；种子发芽10 d为最佳接种时间；子叶—子叶柄垂直接种时再生频率较高；不同品种中,‘浙农8号’再生频率最高,达8089%；最佳生根培养基为1/2 B5+05 mg·L-1 NAA。
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| 关 键 词： | 菜用大豆 离体再生 外植体类型 基因型 |
Optimization of high efficient in vitro regeneration system of vegetable soybean |
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| ZHANG Gu-wen,DING Ju,XU Sheng-chun,LU Xin-miao,HU Qi-zan,GONG Ya-ming.Optimization of high efficient in vitro regeneration system of vegetable soybean[J].Acta Agriculturae Zhejiangensis,2011,23(1):79. |
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| Authors: | ZHANG Gu-wen DING Ju XU Sheng-chun LU Xin-miao HU Qi-zan GONG Ya-ming |
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| Institution: | 1 Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;2 Ninghai Agricultural Technology Extension Station, Ninghai 315600, China |
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| Abstract: | To select the easily regenerating variety and set up a better regeneration system for transgenic work,effects of explant type,hormone concentration,inoculation time,inoculation method and genotype on shoot regeneration were studied based on six varieties of vegetable soybean,including Chunfengzao,Zhenong No.6,Zhenong No.8,Huning 95-1,Liaoxian No.1 and Taiwan 75.The results showed that the regeneration frequency of cotyledon-cotyledon petiole was the highest among different explant types.The best medium of i... |
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| Keywords: | vegetable soybean regeneration in vitro explants type genotype |
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