Occurrence and frequency of putatively Y chromosome linked DNA markers in Cannabis sativa L. |
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Authors: | Giuseppe Mandolino Andrea Carboni Manuela Bagatta VM Cristiana Moliterni Paolo Ranalli |
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Institution: | (1) Istituto Sperimentale per le Colture Industriali, Via di Corticella 133, 40128 Bologna, Italy |
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Abstract: | DNA from female and male hemp (Cannabis sativa L.) plants belonging to nine different varieties were screened with180 RAPD primers in a search for sex-associated DNA markers.
About 1500bands were produced in total, nine primers were found yielding one or two DNA bands amplified in all nine male DNA
bulks and absent in all female DNA bulks. These putatively male-associated markers were then scored in three different F1progenies,
deriving from a cross between a common male parent and three different female plants. The sex of the progeny was accurately
scored on the basis of the floral phenotype, and the presence of the nine male-associated markers was verified by RAPD analysis.
In all three progenies examined, all the male plants showed the DNA markers previously identified by bulk segregant analysis
(BSA) on the hemp varieties, while all the female plants lacked them. The fact that the association between these markers
and the staminate phenotype is found when examining male plants of distantly related varieties, and that such linkage is never
broken when different progenies are examined, strongly supports the hypothesis that the markers found are physically located
on the Y chromosome, in a region excluded from recombination during meiosis. Another marker was shown to be present in the
male parent, in all the male plants of each progeny, and in 50% of the female progenies, while it was absent in the female
parent; the possible occurrence of markers deriving from multiple amplification sites of the genome is discussed.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Cannabis dioecy DNA markers hemp male-specific |
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