| A hybrid swarm population of Pinus densiflora × P. sylvestris inferred from sequence analysis of chloroplast DNA and morphological characters |
| |
| 作者姓名： | Young Hee Joung Jerry L. Hill Jung Oh Hyun Ding Mu Juchun Luo Do Hyung Lee Takayuki Kawahara Jeung Keun Suh Mark S. Roh |
| |
| 作者单位： | Chonnam National University,School of Biological Sciences & Technology,Gwangju,Korea;US Department of Agriculture,Forest Service,Dorena Genetic Resource Center,Washington State University,Pullman,WA,99164,USA;Seoul National University,College of Agriculture and Life Sciences,Seoul,Korea;Chinese Academy of Agricultural Science;Beijing Forestry University,College of Resources and Environment;Yeungnam University,College of Natural Resources,Department of Forest Resources,Gyeongsan,Korea;Hokkaido Research Center,Forestry and Forest Products Research Institute,Sapporo,Japan.;Dankook University,School of Bio-Resource Science,Department of Environment Horticulture,Cheonan,Korea.;US Department of Agriculture,Agricultural Research Service,US National Arboretum,Floral and Nursery Plants Research Unit,Beltsville,MD 20705,USA. |
| |
| 基金项目： | supported by a grant from the Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea (PJ009052) |
| |
| 摘 要： | To confirm a hybrid swarm population of Pinus densiflora × P. sylvestris in Jilin, China, we used needles and seeds from P. densiflora, P. sylvestris, and P. densiflora × P. sylvestris collected from natural stands or experimental stations to study whether shoot apex morphology of 4- year old seedlings can be correlated with the sequence of a chloroplast DNA simple sequence repeat marker (cpDNA SSRs). Total genomic DNA was extracted and subjected to sequence analysis of the pine cpDNA SSR marker Pt15169. Results show that morphological characters from 4-year old seedlings did not correlate with sequence variants of this marker. Marker haplotypes from all P. sylvestris trees had a CTAT element that was absent from all sampled P. densiflora trees. However, both haplotype classes involving this insertion/deletion element were found in a P. densiflora × P. sylvestris population and its seedling progeny. It was concluded that the P. densiflora × P. sylvestris accessions sampled from Jilin, China resulted from bi-directional crosses, as evidenced by both species’ cpDNA haplotypes within the hybrid swarm population.
|
| 关 键 词： | P. sylvestris var. sylvestriformis chloroplast DNA simple sequence repeat sequencing hybrid swarm population |
| 本文献已被 CNKI SpringerLink 等数据库收录！ |
|
