| 携带乙肝大包膜蛋白L基因与结核Esat6融合基因植物表达载体的构建及鉴定 |
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| 引用本文： | 李君武,宫君原,刘鑫,叶秋萍,黄清华. 携带乙肝大包膜蛋白L基因与结核Esat6融合基因植物表达载体的构建及鉴定[J]. 华北农学报, 2011, 26(2): 1-6 |
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| 作者姓名： | 李君武 宫君原 刘鑫 叶秋萍 黄清华 |
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| 作者单位： | 暨南大学医学院,微生物与免疫教研室,广东广州510632 |
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| 基金项目： | 广东省科技计划重大专项，广东省科技计划重点引导项目，国家重大科技专项 |
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| 摘 要： | 构建包含编码结核杆菌Esat6基因和乙肝病毒大包膜蛋白(L蛋白)基因的植物双元表达载体,并转化根癌农杆菌LBA4404.分别以质粒pPIC9K-L和结核杆菌基因组为模板进行PCR扩增,获得L和Esat6基因,然后运用部分重叠聚合酶链式反应扩增出L-Esat6融合基因片段,连接到有玉米特异性启动子globulin-1的p...
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| 关 键 词： | 结核分枝杆菌 乙肝病毒 大包膜蛋白L基因 Esat6基因 globulin-l |
Construction and Identification of the Expression Plasmid Containing Genes Encoding the Large Envelope Protein L of HBV and Esat-6 Gene of Mycobacterium tuberculosis |
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| LI Jun-wu,GONG Jun-yuan,LIU Xin,YE Qiu-ping,HUANG Qing-hua. Construction and Identification of the Expression Plasmid Containing Genes Encoding the Large Envelope Protein L of HBV and Esat-6 Gene of Mycobacterium tuberculosis[J]. Acta Agriculturae Boreali-Sinica, 2011, 26(2): 1-6 |
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| Authors: | LI Jun-wu GONG Jun-yuan LIU Xin YE Qiu-ping HUANG Qing-hua |
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| Affiliation: | LI Jun-wu,GONG Jun-yuan,LIU Xin,YE Qiu-ping,HUANG Qing-hua(Department of Microbiology and Immunology,Medical School of Jinan University,Guangzhou 510632,China) |
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| Abstract: | To construction and identification of the expression plasmid containing genes encoding East6 gene of Mycobacterium tuberculosis and the large envelope protein L of HBV,and transform the recombinant vector into Agrobacterium tumefaciens LBA4404.The L and East-6 gene were amplied from pPIC9K-L and Mycobacterium tuberculosis genome by PCR,then the fusion DNA fragment of L and East6 were amplied by Splicing by Overlap Extension were cloned into the vector pEGG.The combine fragment of promoter globulin-1 and the... |
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| Keywords: | globulin-l |
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