绵羊催产素在渗透泵中稳定性的研究

试验旨在评价39.1℃恒温下绵羊催产素在渗透泵（ALZET^®）中的稳定性。试验分为3个组，分别为渗透泵组、微量离心管组和对照组。除对照组始终冻存于-80℃以外，另外两组置于39.1℃培养箱中进行孵育，分不同时间点采集样品。所有样品经超高效液相色谱串联四级杆/飞行时间质谱（ultra-performance liquid chromatography tandem quadrupole/flight time mass spectrometry，UPLC-Q-TOF MS）方法获得氧化产物提取离子流色谱图，通过比较各产物的峰面积，分析催产素在39.1℃缓释泵中的氧化过程。结果表明，催产素在39.1℃缓释泵及微量离心管中均表现出氧化程度随时间逐渐增加的趋势，两试验组之间差异不显著（P＞0.05）。渗透泵组和微量离心管组不同时间点采集样品中催产素原型占对照组原型的百分比分别为83.6%~117.8%和83.2%~116.2%，说明在渗透泵植入绵羊体内试验中需要考虑因催产素氧化引起的含量变化对试验结果的影响。

Study on the Stability of Sheep Oxytocin in the Osmotic Pump

The experiment was aimed to evaluate the stability of oxytocin in osmotic pump (ALZET^®) at 39.1℃. The experiment was divided into 3 groups, i.e. the osmotic pump group, microcentrifugal tube group and control group. The control group remained frozen at -80℃, the other two groups were incubated in 39.1℃ incubator, and the samples were collected at different time points. All samples were analyzed by ultra-performance liquid chromatography tandem quadrupole/flight time mass spectrometry (UPLC-Q-TOF MS) method. The oxidation process of oxytocin in osmotic pump at 39.1℃ was analyzed by comparing the peak area of main oxidized products extracted ion chromatogram. The results showed that the degree of oxidation increased with time in osmotic pump and microcentrifugal tube at 39.1℃, and there was no significant difference between the two groups (P >0.05). The percentage of oxytocin prototype of samples which collected at different time points of osmotic pump group and microcentrifugal tube group versus control group were 83.6% to 117.8% and 83.2% to 116.2%, respectively, suggested that the trials involved in the osmotic pump implantation of oxytocin in sheep need to consider the effect of content changes caused by oxytocin oxidation on the test results.