基因多态性与超声波活体测定相结合对肉牛主要肉质性状的研究

本试验采用PCR-SSCP与超声波活体测定相结合的方法，对Leptin基因外显子Ⅱ E2JW的SNP位点与宰前/宰后新疆褐牛背膘厚度、眼肌面积、脂肪含量、眼肌高度的部分肉质性状进行关联性分析。结果表明，外显子Ⅱ E2JW位点的PIC处于中度多态（0.25 < PIC < 0.5），E2JW多态位点存在3个基因型：AA、AB和BB，其中AB为优势基因型，B为优势等位基因。相关性结果表明，除预测值中AB、AA基因型个体的肌肉脂肪含量与实测值的分析结果不一致外，其他均表现出新疆褐牛Leptin基因外显子Ⅱ E2JW多态性与预测肉质性状和实测肉质性状关系的一致性。因此，Leptin基因外显子Ⅱ E2JW是影响新疆褐牛肉质性状的候选基因位点，基因多态性结合超声波活体测定技术不仅可以快速、准确地测量部分肉质性状，而且可作为分子标记辅助选择的一种手段。

Study on Genetic Polymorphism and Ultrasound Measurements for Major Meat Quality Traits in Beef Cattle

In this study,we used PCR-SCCP connect with ultrasonic vivo assay techniques, analysis of correlation between Leptin gene exon Ⅱ E2JW polymorphism and part meat quality traits of backfat thickness, eye muscle area, fat content, plot eye height in Xinjiang Brown cattle at ante-mortem, post-mortem. The results showed that Leptin gene exon Ⅱ E2JW site PIC was moderate polymorphisms (0.25 < PIC < 0.5), there were three genotypes of AA, AB and BB, AB genotype were the predominant genotype, B was the predominant allele. In addition, the forecast value of AB and AA gennotypes individual muscle fat content were inconsistent with actual value.Leptin gene exon Ⅱ E2JW was identified the theroy of a candidate gene loci of beef quality traits in Xinjiang Brown cattle.Genetic polymorphism and ultrasound measurements could not only fastly and accurately measure portion meat traits, but also as a means of marker-assisted selection.