葛根对奶牛脂肪肝细胞生化指标的影响

为探讨葛根对奶牛脂肪肝细胞的作用机制，采用胶原酶二步灌流法分离病牛的肝细胞，并观察细胞形态。用不同浓度的葛根水提物处理肝细胞，生化法检测细胞上清液中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）活性，并测定细胞裂解液中甘油三酯（TG）、胆固醇（TC）、丙二醛（MDA）、超氧化物歧化酶（SOD）的活性及含量。结果显示，50、100、500 μg/mL葛根水提物能极显著降低肝细胞中ALT、AST、TG、TC、MDA活性及含量，增加SOD活性（P < 0.01）。葛根水提物能显著改善肝细胞的相关生化指标，其机制可能与调节脂质代谢、抗脂质过氧化作用相关。

Effects of Pueraria on Biochemical Indexes of Fatty Liver Hepatocytes in Dairy Cows

To explore the acting mechanism of Pueraria extract on fatty liver hepatocytes in dairy cows. The hepatocytes in dairy cows were isolated by two-step collagenase perfusion method and observed. The hepatocytes cultured in vitro were treated with different concentrations of Pueraria extract. The activities and contents of ALT, AST, TG, SOD and MDA in the hepatocytes were detected by biochemical methods. Results showed that in the administration of Pueraria extract with the concentrations of 50,100 and 500 μg/mL could extremely significantly reduce the liver cells of ALT, AST, TG, TC, MDA activity and content, increase SOD activity (P < 0.01). The Pueraria extract had significantly therapeutic effect on dairy cows fatty liver of biochemical indexes. The mechanism might be related to regulate lipid metabolism and anti lipid peroxidation.