香猪FSHR基因外显子10的多态性及其与产仔数间的关联分析

促卵泡激素（follicle-stimulating hormone，FSH）是垂体前叶分泌的一种糖蛋白，可通过卵泡刺激素受体（follicle-stimulating hormone receptor，FSHR）调节卵巢的功能。为了研究香猪FSHR基因外显子10的多态性及其与产仔数的关系，试验以从江香猪为研究对象，采用PCR产物直接测序方法检测基因的多态性，分析基因型与香猪产仔数间的关系。对8个候选位点进行群体检测证实其中2个为SNPs位点，分别是rs322800083（C276T）和rs332115220（T601C），且新发现1个SNP位点novel 1（G-92A）；rs322800083和rs332115220位点构成4种单倍型，其中C-T为香猪群体的主要单倍型（45.0%），且在香猪群体中紧密连锁（r²=0.334）。分析4种单倍型与香猪产仔数间的关系，在香猪的二胎产仔数中，单倍型C-T的总产仔数（total number born，TNB）最高，单倍型C-C的TNB最低，两者之间的TNB相差0.99头（P<0.05）；在香猪的三胎产仔数中，单倍型C-T的TNB最高，与单倍型T-C、C-C间的TNB分别相差1.61和2.33头（P<0.01）。rs332115220位点的基因型与香猪的三胎产仔数呈弱的正相关关系（ρ=0.429）。本研究结果提示，单倍型C-T可以作为分子标记应用于香猪产仔数性状的辅助选育。

Polymorphism of FSHR Gene Exon 10 and Its Relationship with the Litter Size of Xiang Pigs

Follicle-stimulating hormone (FSH), a glycoprotein secreted from pituitary, can regulate ovarian function through the FSH receptor (FSHR).To evaluate the effects of FSHR gene on the litter size of Xiang pig population, the polymorphisms of FSHR gene exon 10 were detected by polymerase chain reaction (PCR) and sequencing methods, and the effect of polymorphisms in FSHR gene exon 10 on litter size traits in Xiang pigs was analyzed using the least square analysis.In eight candidate sites, there were two polymorphic loci were detected out from Xiang pig population, which were rs322800083 (C276T) and rs332115220 (T601C). Moreover, a new SNP novel 1 (G-92A) was discovered.Four haplotypes were constructed between rs322800083 and rs332115220 sites, in which C-T was the main haplotype (45.0%) and presented closely linkage disequilibrium (r²=0.334) in Xiang pig population. Furthermore, the haplotype C-T corresponded to the highest total number born (TNB) at the second fetus of Xiang pigs, haplotype C-C to the lowest TNB. It was notified that haplotype C-T produced 0.99 TNB of Xiang pigs more than haplotype C-C (P<0.05). And the TNB at the third fetus was also the highest in haplotype C-T, which produced 1.61 and 2.33 TNB more than haplotypes T-C and C-C (P<0.01). The genotypes of locus rs332115220 was weakly correlated with the third fetus of Xiang pig population (ρ=0.429).The results suggested that haplotype C-T could be used as a molecular marker to assist selection for the litter size of Xiang pigs.