白虎定喘口服液药效研究

为验证新兽药白虎定喘口服液的药效作用，进行了鸡传染性支气管炎病毒（infectious bronchitis virus，IBV）鸡胚增殖抑制试验、气管纤毛运动试验及SPF雏鸡人工感染IBV疗效试验。将试验药液与等量IBV液混合作用后，接种11日龄SPF鸡胚，37℃孵育168 h，取存活鸡胚肾脏进行IBV的RT-PCR检测，结果表明，药物组168 h鸡胚存活率为80%，阳性对照组为20%，两组间差异显著（P<0.05）；试验药物组RT-PCR检测无目的条带出现，而阳性对照组有目的条带出现。40日龄SPF鸡连续用药3 d后，气管内注入0.02 mL墨汁，测量1 min内墨汁移动的距离，结果表明药物组墨汁移动距离极显著大于生理盐水对照组（P<0.01）。17日龄SPF鸡用IBV-M41滴鼻，0.3 mL/只，24 h后开始灌服药液，1 mL/kg，连用5 d，结果表明，试验药物组与攻毒对照组在临床症状计分、痊愈率上均存在显著差异（P<0.05）；试验组气管黏膜组织切片未见明显病变，而阳性对照组有明显病理变化。以上试验结果表明，白虎定喘口服液对IBV具有较好的体外抑制作用，可显著促进气管纤毛的运动，对鸡传染性支气管炎（IB）具有较好的临床防制效果。

Study on Efficacy of Baihu Dingchuan Oral Liquid

In order to verify the effect of Baihu Dingchuan oral liquid of new veterinary drugs,the effects of infectious bronchitis virus (IBV) on the proliferation inhibition test, tracheal cilia movement test and SPF chickens infected with IBV were tested.The test solution was mixed with an equal amount of IBV solution,inoculation of 11-day-old SPF chicken embryo,incubation at 37℃ for 168 h.RT-PCR was performed to detect IBV in kidney of live chick embryo.The results showed that the survival rate of 168 h chicken embryo was 80% in the drug group,but the positive control group was 20%, and there was significant difference between two groups (P<0.05). In the test drug group, no band was detected by RT-PCR, but the target band appeared in the positive control group.After 40 days old SPF chickens were administered continuously for 3 days, 0.02 mL of ink was injected into the trachea. The distance of ink movement in 1 min was measured. The results showed that the migration distance of ink was extremely significantly higher than that of saline control group (P<0.01).In the 17-day-old SPF chickens, IBV-M41 was infused into the nasal mucosa with 0.3 mL. After 24 h, 1 mL/kg was administered for 5 days. The results showed that there were significant differences in clinical symptom score and cure rate between the test drug group and the control group (P<0.05). There was no significant pathological changes in the tracheal mucosa of the experimental group, but the pathological changes in the positive control group were significant. The above results showed that Baihu Dingchuan oral liquid had good inhibitory effect on IBV in vitro, which could promote the movement of tracheal cilia, and had good clinical control effect on infectious bronchitis.