中药犬螨灵对犬蠕形螨的疗效试验

为筛选具有显著治疗犬蠕形螨的中药制剂，试验将中药小茴香、蛇床子、羊蹄根采用水煮醇沉及蒸馏等提取方法制备成中药制剂犬螨灵，并观察其对犬蠕形螨的疗效。将犬螨灵设为高、中、低3个浓度，其生药含量分别为2.0、1.0和0.5 g/mL，采用点滴法进行体外抑杀蠕形螨试验，在此基础上对12只接种蠕形螨的试验犬进行治疗试验，为验证治疗效果，对临床上已确诊的45只患有蠕形螨的病犬分3组进行临床治疗试验。结果显示，中药犬螨灵3种剂量均有较强的体外抑杀螨虫作用，高剂量组作用后4 h即可完全抑杀蠕形螨，1%伊维菌素对照组则为作用后8 h；高剂量组在治疗3周后，患处有大量新毛生长，红疹结节和皮屑消失，患处皮肤已痊愈，1%伊维菌素治疗组患犬皮肤好转，仅有少量新毛生长，不能达到痊愈标准；高、中剂量组皮肤刮取物螨虫数量平均比例及停药1个月后复发率均为0，1%伊维菌素治疗组皮肤刮取物螨虫数量平均比例及停药1月后的复发率分别为10.63%和9.17%。高剂量中药犬螨灵与1%伊维菌素两种药物联合治疗组，对临床上患病犬进行3周治疗，其虫卵平均转阴率、治愈率与平均治愈天数分别为100.00%、93.33%和19.56 d，其效果均优于中药犬螨灵与1%伊维菌素单独治疗组。结果表明，高剂量自制中药制剂犬螨灵对犬蠕形螨体外抑杀作用、接种蠕形螨试验犬与临床自然感染病例均有显著的治疗效果，优于1%伊维菌素对照组，犬螨灵作为中药杀螨虫制剂具有较好的市场开发前景。

Efficacy of Chinese Herbal Medicine Canine Acariasis Spirit on Demodex canis

In order to screen the Chinese herbal medicine for the treatment of Demodex canis, canine acariasis spirit was prepared by Foeniculum vulgare, Fructus cnidii and Bauhinia by water alcohol precipitation and distillation extraction methods. To observe the effect on Demodex canis, canine acariasis spirit was set to high, medium and low concentrations, the content of crude drug were 2.0, 1.0 and 0.5 g/mL, respectively. The experiment of killing Demodex canis was carried out by drop method. On this basis, 12 dogs inoculated with Demodex canis were treated. In order to verify the therapeutic effect, 45 clinically diagnosed dogs with Demodex canis were divided into three groups. The results showed that 3 doses of the traditional Chinese medicine canine acariasis spirit all had strong inhibition effect to kill mites, the high dose group could completely kill Demodex canis only 4 h after treatment, the action of 1% ivermectin control group was 8 h after treatment; 3 weeks later, a large number of new hair grew, rash and skin nodules disappeared, the skin lesion had healed in the affected area of high dose group, in 1% ivermectin treatment group, the dog skin improved, only a small amount of new hair grew, it couldn't cure standard; In the high and middle dose group, the average number of the ratio of skin scraping mites and the recurrence rate after drug withdrawal was 0 in one month later; The average number of mites proportion and recurrence rates of skin scrapings in 1% ivermectin treatment group were 10.63% and 9.17% in one month later; After 3 weeks of treatment, the total average eggs negative rate, cure rate and average cure days were 100.00%, 93.33% and 19.56 d in high dose traditional Chinese medicine canine acariasis spirit and 1% ivermectin combined treatment group, the results were better than the traditional Chinese medicine canine acariasis spirit and 1% ivermectin alone treatment group. The results showed that the effect of the high dose group self-made Chinese medicine canine acariasis spirit on killing Demodex canis in vitro, inoculation of Demodex canis and clinical therapeutic effect of natural infection cases were significantly better than 1% ivermectin control group, canine acariasis spirit as a traditional Chinese medicine preparation would have a good market development prospects.