马身猪耳缘组织成纤维细胞的体外培养与鉴定

为研究马身猪体细胞的生物学特性，试验以3日龄马身猪耳缘组织为材料，采用组织块贴壁法建立马身猪耳缘组织成纤维细胞系，并对体外获得的细胞系进行生物学特性检测。结果表明，获得的马身猪耳缘组织成纤维细胞系符合成纤维细胞的基本特征，细胞贴壁生长，呈典型的梭形、星形和多边形，细胞汇合成单层的时间为10~13 d，生长曲线呈S型，中期染色体二倍体（2n=38）占主体，约占细胞总数的84%，符合细胞建系的要求；脂质体（Lipofectamine^TM 2000）转染绿色荧光蛋白质粒（pEGFP-C1）的转染效率为25%。马身猪耳缘组织成纤维细胞系的成功建立为地方猪种遗传资源的保护开辟了新的方法。

In vitro Culture and Identification of Ear Marginal Fibroblast Cell Lines Derived from Mashen Pigs

In order to study the biological characteristics of somatic cells of Mashen pig, the ear marginal fibroblast cell lines of Mashen pig were established from 3 days old Mashen pig by direct explant method,and the biological characteristics of cell lines were detected. The results showed that the cell lines obtained from Mashen pig were in conformity with basic properties of typical fibroblasts, the shape of adherent cells were typical spindle, star and polygon, the time of grew into monolayer cells was 10 to 13 d, the growth curve was typical S shaped. The frequency of metaphase chromosome number (2n=38) was about 84% of the total number of cells, and it had met the establishment standard of fibroblast cell line. The transfection efficiency of green fluorescent protein(pEGFP-C1)transfected by lipofectamine-mediated method was 25%. The successful establishment of the fibroblast cell lines of Mashen pig had opened up a new method for the conservation of genetic resources.