哺乳动物生殖细胞冷冻保存对其DNA甲基化影响的研究进展

哺乳动物生殖细胞和胚胎冷冻保存在辅助生殖技术中得到了广泛的应用，同时在保存物种的多样性方面发挥着巨大的作用。但是，冷冻引起的DNA甲基化对其印记基因缺陷性疾病的发生和后代生理功能的差异会有很大的影响。作者就冷冻保存技术对配子（精子和卵子）和胚胎的DNA甲基化的影响以及冷冻引起的DNA甲基化对后代的影响进行了综述，以期对配子的冷冻保存和辅助生殖技术的发展提供参考。

Research Progress on the Relationship between Cryopreservation of Mammalian Germ Cells and Their DNA Methylation

Cryopreservation of mammalian germ cells and embryos has been widely used in assisted reproduction. It also plays an important role in preservation of species diversity. DNA methylation abnormality induced by freezing in germ cells or embryos has an significant impact on the occurrence of disease and the physiological function of the offspring. In this paper, we summarize the effects of cryopreservation of mammalian germ cells and embryos on their pattern of DNA methylation which in turn make the developmental deficiency in the progeny from these gametes or embryos. Finally, it will provide the reference on the gamete cryopreservation and the development of assisted reproductive technology.