蜂花粉醇提物对衰老小鼠学习、记忆及脑组织抗氧化能力的影响

试验旨在探讨蜂花粉醇提物（ethanol extracts from bee pollen，EEBP）对D-半乳糖诱导的衰老雄性小鼠学习、记忆和脑组织抗氧化能力的影响。以昆明种雄性小鼠为研究对象，按体重随机分为正常对照组、模型对照组和低、中、高剂量（400、800、1 600 mg/kg体重）EEBP组。除正常对照组外，其余各组小鼠每天颈背部皮下注射500 mg/kg体重 D-半乳糖0.2 mL，正常对照组注射等体积的生理盐水。EEBP组小鼠每天灌胃0.3 mL EEBP悬浮液，对照组小鼠灌胃等体积的蒸馏水，试验期56 d。采用Y型迷宫和跳台仪测试小鼠学习记忆能力，同时测定各组小鼠脑组织超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、过氧化氢酶（catalase，CAT）的活力及丙二醛（ma1ondialdehvde，MDA）的含量。结果表明，EEBP能显著降低D-半乳糖诱导的衰老小鼠Y型迷宫的学习次数，提高正确次数，延长跳台潜伏期，降低错误次数；提高衰老模型小鼠脑组织SOD、GSH-Px和CAT活性，降低MDA含量。以上结果说明EEBP可以改善D-半乳糖致衰老模型小鼠学习、记忆能力，提高脑组织的抗氧化能力，具有一定延缓脑衰老的作用。

Effects of Ethanol Extracts from Bee Pollen on Learning,Memory and Brain Antioxidant Abilities in Aged Mice

The experiment was aimed to investigate the effects of ethanol extracts from bee pollen (EEBP) on learning, memory and brain antioxidant abilities in aged mice induced by D-galactose. Fifty ICR mice were randomly divided into five groups, designated as normal control, model control, low-dose, moderate-dose and high-dose EEBP groups. The mice in normal control group were given saline by gavage, in the other groups were given D-galactose solution by neck back subcutaneous injection at the daily dose of 500 mg/kg·BW for 8 consecutive weeks. At the same time,EEBP treated mice were administered EEBP by gavage at the daily doses of 400,800 and 1 600 mg/kg·BW, respectively. The learning and memory abilities of mice were determined by Y-maze and step-down tests. The content of malondialdehyde (MDA) and activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in brain tissue of the mice were measured. The results showed that EEBP could enhance the memory, increase the activities of SOD,GSH-Px and decrease the content of CAT in mice.In conclusion, EEBP could ameliorate the degeneration of learning and memory abilities in D-galactose-induced aging mice,improve brain antioxidant abilities and thus delay brain aging.