刺糖对对乙酰氨基酚诱导的肝损伤的保护作用

对乙酰氨基酚（N-acetyl-para-aminophenol，APAP）过量使用能够引起肝脏损伤，本研究旨在探讨刺糖对APAP所致小鼠急性肝损伤的保护作用及潜在的作用机理。采用水提法提取刺糖的有效成分，通过苯酚硫酸法和铝盐显色法进行刺糖提取物成分鉴定；49只雌性小鼠随机分为7组，正常对照组、模型组、刺糖不同浓度的提取物（600、300和150 mg/kg体重）+APAP组、水飞蓟宾阳性对照组及刺糖对照组（600 mg/kg体重），灌胃3 d，末次给药1 h后，腹腔注射APAP 300 mg/kg体重，24 h后处死小鼠并采集血样和肝脏组织样品。检测血清谷丙转氨酶（ALT）和谷草转氨酶（AST）的活性；用HE染色制作病理标本，观察肝脏病理学变化。结果显示，刺糖提取物能够极显著降低小鼠血清中AST、ALT的含量（P<0.01），APAP所引起的病理变化明显改善。本研究结果表明，刺糖对APAP所致的小鼠急性肝损伤有一定的保护作用。

Protective Effect of Saccharum alhagi Against N-acetyl-para-aminophenol induced Liver Injury in Mice

N-acetyl-para-aminophenol (APAP) overdose use induced liver damage.This study was aimed to evaluate the hepatoprotective effect of Saccharum alhagi against APAP-induced liver injury in mice. The aqueous extract of Saccharum alhagi was prepared, ant its ingredients were measured by phenol-sulfuric acid method and aluminium salt chromogenic method. The mice were randomly divided into seven groups,including normal control group,APAP model group,Saccharum alhagi (150, 300 and 600 mg/kg·BW)+APAP groups,positive control group and Saccharum alhagi control group. Administration once per day for 3 consecutive days,with 300 mg/(kg·BW) of APAP after 1 h from the last administration of Saccharum alhagi. 300 mg/(kg·BW) APAP were used to induce liver injury, and after 24 h from APAP challenge,the experimental animals were sacrificed to collect blood and liver tissue samples. The level of serum transaminase (ALT and AST) and the liver pathological changes were observed. The results indicated that the levels of serum AST and ALT were extremely significant lower in the Saccharum alhagi treated groups than the APAP treated group (P<0.01), and the pathological changes were significantly improved. This study showed that Saccharum alhagi could be an effective therapeutic source in APAP-induced liver injuries in mice.