拜城油鸡公鸡A-FABP基因多态性及其与生产性能的相关性分析

试验旨在研究拜城油鸡公鸡中脂肪型脂肪酸结合蛋白（adipocyte fatty acid binding protein，A-FABP）基因的多态性及其与生产性能的相关性，寻找可用于拜城油鸡选育的分子遗传标记。随机选取92只6周龄拜城油鸡公鸡，采用PCR-RFLP方法检测A-FABP基因的多态性。每种基因型选取15只体重相近的个体随机分为3组，每组每种基因型个体各5只，分别在12、18、24周龄时屠宰并对生产性能指标进行测定。结果显示，在拜城油鸡公鸡中检测到A-FABP基因有3种基因型：AA、AB、BB，BB为优势基因型，B为优势等位基因，不符合哈代-温伯格平衡定律（P<0.01）。12周龄时，各基因型间各生产性能指标差异均不显著（P>0.05）；18周龄时，BB基因型肌间脂宽显著高于AA基因型（P<0.05），BB和AB基因型腹脂重极显著高于AA基因型（P<0.01），腹脂率显著高于AA基因型（P<0.05）；24周龄时，BB基因型体重、肌间脂宽、腹脂重均显著高于AA基因型（P<0.05）。推测A-FABP基因可以作为与拜城油鸡公鸡脂肪性状相关的候选标记之一。

Polymorphism of A-FABP Gene and Its Association Analysis with the Productive Performance in Male Baicheng Fatty Chicken

In order to search the molecular genetic markers for breeding of Baicheng fatty chicken, the relationship between the polymorphism of adipocyte fatty acid binding protein (A-FABP) gene and the productive performance of the male chickens at different ages was studied. Ninety two chickens with age of 6 weeks were randomly selected. The polymorphism of A-FABP gene was detected by PCR-RFLP method. In each genotype group, fifteen chickens with similar body weight were categorized, each group was randomly divided into three sub-groups with equal numbers. The productive performance of the chickens was measured at their age of 12, 18 and 24 weeks. The results showed that there were three genotypes of A-FABP gene:AA,AB and BB, BB genotype was the dominant genotype,allele B was the advantageous allele,and it was not in Hardy-Weinberg equilibrium (P<0.01). At the age of 12 weeks,no significant difference was found among the measured productive performance (P>0.05). At the age of 18 weeks, it was found that the intramuscular fat width of BB genotype was significantly higher than AA genotype (P<0.05), the abdominal fat weight of BB and AB genotypes were extremely significantly higher than AA genotype (P<0.01),and the abdominal fat weight percentage of BB and AB genotypes were significantly higher than AA genotype (P<0.05). At the age of 24 weeks, it was found that the body weight, intramuscular fat width and abdominal fat weight of BB genotype were significantly higher than that of AA genotype (P<0.05). Therefore, this results suggested that A-FABP gene could be used as a potential candidate marker for the association of fatty traits in male Baicheng fatty chicken.