MYLPF基因在肉牛肌肉生长过程中的功能研究

试验旨在探究快肌肌浆球蛋白可调节磷酸化轻链（myosin light chain，phosphorylatable，fast skeletal muscle，HUMMLC2B或MYLPF）基因在布莱凯特黑牛各组织中的表达情况，以及布莱凯特黑牛与鲁西黄牛背最长肌中MYLPF基因表达量的变化规律，为探讨其与肌肉生长发育关系奠定基础。以布莱凯特黑牛和鲁西黄牛为研究对象，利用实时荧光定量PCR检测其背最长肌、心脏、肺脏、肝脏、脾脏等10个组织中MYLPF基因的表达情况，并对布莱凯特黑牛和鲁西黄牛2、6、10和12月龄4个不同时期背最长肌中MYLPF基因的表达量变化进行研究。结果表明，MYLPF基因在背最长肌中高表达，在心脏中少量表达，在其他组织中几乎不表达。MYLPF基因在2、6、10和12月龄牛背最长肌中均高表达，但随着年龄的增加MYLPF基因的表达量逐渐降低，2月龄的表达量最高，极显著高于其他3个时期（P<0.01），12月龄表达量最低。4个时期布莱凯特黑牛背最长肌中MYLPF基因的表达量均高于鲁西黄牛，其中10月龄差异极显著（P<0.01），2和12月龄差异显著（P<0.05），6月龄差异不显著（P>0.05）。综上结果表明，背最长肌中MYLPF基因可能对骨骼肌生长发育起调节作用，可为研究肉牛骨骼肌生长发育机理和阐明肌肉生长的分子机制提供参考。

Study on the Function of MYLPF Gene of Muscle Growth in Cattle

This study was aimed to investigate the expression level of myosin light chain,phosphorylatable,fast skeletal muscle (MYLPF) gene in the tissues of Black cattle, and its expression level in the longissimus dorsi muscle of Black cattle and Luxi cattle, and to lay the foundation for the study of its relationship with muscle growth and development. The MYLPF gene expression level in 10 tissues of longissimus dorsi, heart, lung, liver and spleen and so on were detected by Real-time quantitative PCR, and then the changes of MYLPF gene expression level in longissimus dorsi of Black cattle and Luxi cattle at 2, 6, 10 and 12 months of age were studied. The results showed that MYLPF gene was highly expressed in the longissimus dorsi, lowly expressed in the heart, and the other tissues were almost not expressed. The MYLPF gene expression level in the longissimus dorsi was high in 2, 6, 10 and 12 months, with the increase of age,the MYLPF gene expression level decreased gradually, the expression level of 2 months of age was the highest, which was extremely significantly higher than that of the other three periods (P<0.01). The MYLPF gene expression level in the longissimus dorsi of Black cattle was higher than that of Luxi cattle in four period, which in 10 months of age were extremely significantly different (P<0.01), 2 and 12 months of age had significant difference (P<0.05), the difference in 6 months of age was not significant (P>0.05). The results suggested that MYLPF gene in the longissimus dorsi might play a regulatory role in the growth and development of skeletal muscle, which might provide a reference for studying the mechanism of growth and development of skeletal muscle and the molecular mechanism of muscle growth.